FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_67.3520000005dee4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_67.3520000005dee4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14946
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA1280.8564164324902984No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC1160.776127391944333No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA1100.7359828716713501No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1090.7292921182925197No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA870.5820955439582497No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA840.5620232838217584No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA840.5620232838217584No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA790.528569516927606No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA770.5151880101699451No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.4951157500334538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.4683527365181319No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA680.454971229760471No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA660.4415897230028101No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA640.4282082162451492No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA560.3746821892145055No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA560.3746821892145055No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA540.3613006824568446No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA500.3345376689415228No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA500.3345376689415228No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA490.32784691556269235No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC460.307774655426201No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA450.3010839020473705No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA430.2877023952897096No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA410.2743208885320487No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA390.2609393817743878No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA390.2609393817743878No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA380.25424862839555734No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA380.25424862839555734No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT370.2475578750167269No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA370.2475578750167269No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA350.23417636825906596No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA340.2274856148802355No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA320.2141041081225746No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA290.19403184798608325No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA270.1806503412284223No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA260.17395958784959187No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA260.17395958784959187No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA260.17395958784959187No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA260.17395958784959187No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT250.1672688344707614No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC250.1672688344707614No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA250.1672688344707614No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA240.16057808109193095No Hit
ACGCAGAGTGAATGGGCTCAAAAAAAAAAAAAA240.16057808109193095No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA240.16057808109193095No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA240.16057808109193095No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA240.16057808109193095No Hit
TATCAACGCAGAGTGAATGGGCTCAAAAAAAAA230.1538873277131005No Hit
GAGTGAATGGGCTCAAAAAAAAAAAAAAAAAAA230.1538873277131005No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG220.14719657433427003No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA220.14719657433427003No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA210.1405058209554396No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA210.1405058209554396No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA200.13381506757660913No Hit
CAACGCAGAGTGAATGGGCTCTGAAAAAAAAAA180.12043356081894822No Hit
CAACTCAGAGTGAATGGGCTCTGAAAAAAAAAA180.12043356081894822No Hit
TATCTACGCAGAGTGAATGGGCTCTGAAAAAAA180.12043356081894822No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11374280744011775No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA170.11374280744011775No Hit
AACGCAGAGTGAATGGGCTCAAAAAAAAAAAAA170.11374280744011775No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA170.11374280744011775No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA170.11374280744011775No Hit
CTGAACGTACTCAAAAAAAAAAAAAAAAAAAAA170.11374280744011775No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA170.11374280744011775No Hit
GTGAATGGGAATGCGCTAAAAAAAAAAAAAAAA160.1070520540612873No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA160.1070520540612873No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA160.1070520540612873No Hit
GCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1070520540612873No Hit
ACGCAGAGTGAATGGGAATGCGCTCAAAAAAAA160.1070520540612873No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA160.1070520540612873No Hit
CAATGCGCTCTGAACGTACTCTGCGTTGATACC150.10036130068245684No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA150.10036130068245684No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA150.10036130068245684No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC150.10036130068245684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00837482626.9797951
GTATCAA450.001046956717.9865321
TATCAAC1002.1313099E-816.187881
GCGTTGA1355.4636985E-813.03415927
ATCAACG1253.2892967E-712.9503032
TCAACGC1358.3766645E-711.9910223
CAACGCA1502.986184E-610.7919194
CAATGCG1200.00642422358.9932668
TGCGTTG2008.7506305E-68.79805826
AACGCAG2051.2497916E-58.554575
TCTGCGT3508.185452E-118.50801227
CTGCGTT2202.914607E-57.99823425
CGCAGAG2851.464703E-67.57327657
CGCTCTG4102.2719178E-97.238482513
ACGCAGA2809.705038E-67.2267326
GCGCTCT4152.904926E-97.151272312
GCAGAGT2704.884763E-56.99476248
CTCTGCG4305.5497367E-96.925125626
CAGAGTG2859.957224E-56.6266179
TGAATGG4704.7639332E-96.601442
Produced by FastQC (version 0.11.2)