FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_62.3520000005de44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_62.3520000005de44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9063
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA1751.9309279488028248No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC1511.6661149729670088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.6178969436169038No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA530.5847953216374269No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA440.48549045569899596No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA430.4744565817058369No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA430.4744565817058369No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA410.45238883371951893No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC370.4082533377468829No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA340.3751517157674059No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA310.34205009378792894No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA300.33101621979477No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA300.33101621979477No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA290.31998234580161095No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA290.31998234580161095No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC280.3089484718084519No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.29791459781529295No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT270.29791459781529295No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA270.29791459781529295No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC240.264812975835816No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA230.25377910184265695No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA220.24274522784949798No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.24274522784949798No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA210.23171135385633895No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA210.23171135385633895No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA210.23171135385633895No Hit
ACGCAACAGAGCATACGTACTCTGCGTTGATAC200.22067747986317995No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA200.22067747986317995No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA180.19860973187686196No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA170.18757585788370296No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA170.18757585788370296No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA170.18757585788370296No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA170.18757585788370296No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA160.17654198389054399No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA160.17654198389054399No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA160.17654198389054399No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA150.165508109897385No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA150.165508109897385No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT150.165508109897385No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA140.15447423590422596No Hit
ACAGAGCATACGTACTCAAAAAAAAAAAAAAAA140.15447423590422596No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.15447423590422596No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA140.15447423590422596No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA130.14344036191106696No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.14344036191106696No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA130.14344036191106696No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA130.14344036191106696No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA120.132406487917908No Hit
TCGCAACAGAGCATACGTACTCTGCGTTGATAC120.132406487917908No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA120.132406487917908No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA110.12137261392474899No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA110.12137261392474899No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC110.12137261392474899No Hit
GAGTGAATGGGGCAACAGAGAAAAAAAAAAAAA110.12137261392474899No Hit
TATCAACGCAGAGTGAATGGGCAACAGAAAAAA110.12137261392474899No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT110.12137261392474899No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA110.12137261392474899No Hit
CAGCAACAGAGCATACGTACTCTGCGTTGATAC110.12137261392474899No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAA100.11033873993158998No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA100.11033873993158998No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.11033873993158998No Hit
AACGCAGAGTGAATGGGCAACAGAGCATAAAAA100.11033873993158998No Hit
GAGCAACAGAGCATACGTACTCTGCGTTGATAC100.11033873993158998No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.11033873993158998No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA100.11033873993158998No Hit
GGCAACAGAGCATACGTACTCTGCGTTGATACC100.11033873993158998No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC300.002099422722.49539427
GATACCA350.00513439119.28176726
TGATACC350.00513439119.28176725
TATCAAC603.4795492E-415.8342591
CGTTGAT802.0072443E-413.49723827
AATGGGG1101.0022995E-613.4972374
GCAGAGT958.573001E-411.3660963
TGAATGG2353.45608E-1110.912662
GTGAATG2507.2759576E-1210.8577781
GCGTTGA1000.001317958810.7977926
ACGCAGA1153.2281812E-410.6217391
CGCAGAG1205.044719E-410.1229282
GAATGGG2602.1827873E-109.8633663
TGCGTTG1100.00291746379.81617325
CAGAGTG1200.0059935218.99815754
CTGCGTT1200.0059935218.998157524
AGAGTGA1250.0083855988.6382325
AGTGAAT1550.005222497.83710587
GAGTGAA1650.0091591267.362136
Produced by FastQC (version 0.11.2)