FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_6.3520000005d74b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_6.3520000005d74b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34031
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC4661.3693397196673622No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC1490.4378360906232553No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1290.3790661455731539No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA1130.3320501895330728No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC1110.3261731950280627No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1060.31148070876553735No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1020.299726719755517No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA900.2644647527254562No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT830.24389527195792074No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA780.22920278569539537No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA770.2262642884428903No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA740.2174487966853751No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA670.1968793159178396No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA590.17337133789779907No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC580.17043284064529401No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA580.17043284064529401No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA570.16749434339278893No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.1616173488877788No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA540.15867885163527373No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC510.1498633598777585No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA490.1439863653727484No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT450.1322323763627281No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA430.12635538185771797No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA430.12635538185771797No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA400.11753989010020276No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA400.11753989010020276No Hit
GTGAATGGGTGCTACGAAAAAAAAAAAAAAAAA400.11753989010020276No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA390.11460139284769769No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA380.11166289559519263No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT380.11166289559519263No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA360.10578590109018247No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA350.10284740383767742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC806.002665E-1122.03846227
GATACCA851.3278623E-1020.74208326
TGATACC1052.25009E-916.79120825
TTGATAC1054.1600288E-815.49957724
GTTGATA1107.365634E-814.79505223
TATCAAC2400.012.9205881
ACTGCTA956.790012E-512.7727567
AATGGGT956.790012E-512.7727564
ATCAACG2850.011.35355952
TCAACGC2950.010.9686953
TCTGCGT5100.010.63696527
AGTACGT1050.002264319210.2722682
CAACGCA3050.010.1670214
GTATCAA2451.6589183E-99.9054021
GAGTACG1100.00333086689.8053471
CGTTGAT1701.41450655E-59.57326922
GCGTTGA1854.1194297E-58.7453121
AACGCAG3653.45608E-118.49572855
CTCTGCG6500.08.34592626
GTGAATG7000.07.7042011
Produced by FastQC (version 0.11.2)