FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_56.3520000005dd88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_56.3520000005dd88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA3593.164668547249647No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA1191.0490126939351199No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC790.6964033850493653No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC730.6435119887165022No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA690.6082510578279267No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA570.5024682651622003No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA480.4231311706629055No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA450.3966854724964739No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC420.3702397743300423No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA420.3702397743300423No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA410.36142454160789844No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC410.36142454160789844No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.3526093088857546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.31734837799717913No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA350.3085331452750353No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA310.2732722143864598No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA280.2468265162200282No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA260.22919605077574046No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA260.22919605077574046No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA250.2203808180535966No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC250.2203808180535966No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA240.21156558533145275No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG210.18511988716502115No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA210.18511988716502115No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA200.1763046544428773No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA200.1763046544428773No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC190.16748942172073342No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC190.16748942172073342No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT180.15867418899858957No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA180.15867418899858957No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA170.14985895627644572No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA160.14104372355430184No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA150.13222849083215796No Hit
CGCATGAGCACTGTACGTACTCTGCGTTGATAC150.13222849083215796No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA150.13222849083215796No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA150.13222849083215796No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC150.13222849083215796No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1234132581100141No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA140.1234132581100141No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA140.1234132581100141No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA140.1234132581100141No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA140.1234132581100141No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA130.11459802538787023No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA130.11459802538787023No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA130.11459802538787023No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT130.11459802538787023No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA120.10578279266572638No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA120.10578279266572638No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA120.10578279266572638No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA120.10578279266572638No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA120.10578279266572638No Hit
GTGAATGGGGCATGAGCAAAAAAAAAAAAAAAA120.10578279266572638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA702.1100277E-1023.30357227
TGATACC755.002221E-1021.7526
GTATCAA703.3671495E-617.3230081
TATCAAC756.500062E-616.1681422
ATCAACG801.2001334E-515.1576333
TTGATAC1105.799302E-814.82954625
CAACGCA852.1308146E-514.2660075
GTTGATA1201.6854028E-713.5937524
AACGCAG802.1114651E-413.4734526
TCAACGC903.6543537E-513.4734514
CGTTGAT1252.7746682E-713.05000123
ACGCAGA1200.0062866198.9823011
GCGTTGA1555.0207163E-48.77016122
CGCAGAG1450.0030235748.3628328
TGCGTTG2050.0010361237.22965721
TCTGCGT3103.2920558E-56.577620527
CTGCGTT2300.00318693356.501358526
GTGAATG2450.0067917026.04930451
TGAATGG2500.0083796075.9283192
CTCTGCG3501.7736795E-45.825892426
Produced by FastQC (version 0.11.2)