FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_54.3520000005dd38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_54.3520000005dd38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC1081.8940722553490004No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC410.7190459487898984No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA320.5612065941774815No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA320.5612065941774815No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA290.5085934759733427No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC270.4735180638372501No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA250.4384426517011574No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA240.4209049456331112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.4033672395650649No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.4033672395650649No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA220.3858295334970186No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA220.3858295334970186No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA210.36829182742897226No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT200.350754121360926No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA190.3332164152928797No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT130.22799017888460188No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA130.22799017888460188No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA130.22799017888460188No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT120.2104524728165556No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA110.1929147667485093No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC110.1929147667485093No Hit
GTGAATGGGGAGTACGTCTGATCGTAGCAAAAA90.1578393546124167No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA90.1578393546124167No Hit
GTGAATGGGATCAGACGTACTCAAAAAAAAAAA90.1578393546124167No Hit
GAGTGAATGGGGTCTGATCGTAGCAAAAAAAAA90.1578393546124167No Hit
GTGAATGGGGTCTGATCGTAGCAAAAAAAAAAA90.1578393546124167No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT80.14030164854437038No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA80.14030164854437038No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA80.14030164854437038No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC80.14030164854437038No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG70.1227639424763241No Hit
ACGCAGAGTGAATGGGGAGTACGTCTGATCGTA70.1227639424763241No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA70.1227639424763241No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA70.1227639424763241No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GTGAATGGGGGAGTGCAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA60.1052262364082778No Hit
GTGAATGGGGCCGTGCAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CTCCGTGGCTAGTCTGCGCTCTTTGGACTTTGA60.1052262364082778No Hit
GTGAATGGGGCAGTGCAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GTGAATGGGTGCTACGAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
CCCATGCTACGATCAGACGTACTCTGCGTTGAT60.1052262364082778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC500.001925680816.168421
AATGGGA600.00649296313.4736844
GTGAATG1602.3888788E-811.7894741
TGAATGG1602.3888788E-811.7894742
GAATGGG1604.5342167E-610.1052633
TCTGCGT1200.00510540659.06194727
Produced by FastQC (version 0.11.2)