FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_52.3520000005dd01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_52.3520000005dd01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4081
Sequences flagged as poor quality0
Sequence length33
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA982.401372212692967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.5390835579514826No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA130.31854937515314874No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA120.294045577064445No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC110.2695417789757413No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA100.24503798088703752No Hit
GTTCATAAATAAATAAAAATGTATACAATGTTT100.24503798088703752No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA100.24503798088703752No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA90.22053418279833373No Hit
TCCTAATTTCGTGCACAGTTCATAAATAAATAA80.19603038470963No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA70.17152658662092624No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC70.17152658662092624No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA60.1470227885322225No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA60.1470227885322225No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA50.12251899044351876No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA50.12251899044351876No Hit
GTATATGATGTTAATAATGTTGTAATAATCGGA50.12251899044351876No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA50.12251899044351876No Hit
AATGTATACAATGTTTGTAAAAAAAAAAAAAAA50.12251899044351876No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC50.12251899044351876No Hit
GAGTGAATGGGTATCGAAAAAAAAAAAAAAAAA50.12251899044351876No Hit
GTATACAATGTTTGTAAAAAAAAAAAAAAAAAA50.12251899044351876No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA50.12251899044351876No Hit
TATCAACGCAGAGTGAATGGGTATCGACAAAAA50.12251899044351876No Hit
ACGCAGAGTGAATGGGTATCGAAAAAAAAAAAA50.12251899044351876No Hit
ACCTTGTTGTGTGTTGTTACTAATGTTGTAATA50.12251899044351876No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA50.12251899044351876No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)