FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_5.3520000005d721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_5.3520000005d721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32710
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG2120.6481198410272088No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1260.3852033017425864No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC930.2843167227147661No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT900.2751452155304189No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC820.25068786303882606No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA740.22623051054723328No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA680.20788749617853866No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA670.20483032711708957No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA620.18954448180984407No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA560.1712014674411495No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA550.1681442983797004No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA510.155915622133904No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA490.14980128401100581No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.14980128401100581No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA490.14980128401100581No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA430.13145826964231122No Hit
GCCTAGACGTAGCTCACGTACTCTGCGTTGATA370.11311525527361664No Hit
GTGAATGGGGTAGACGTAAAAAAAAAAAAAAAA350.10700091715071844No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA340.10394374808926933No Hit
CATAGACGTAGCTCACGTACTCTGCGTTGATAC330.10088657902782024No Hit
GCCCTAGACGTAGCTCACGTACTCTGCGTTGAT330.10088657902782024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACTG356.212622E-627.06441727
TACCACT356.212622E-627.06441726
GTATCAT200.0085189526.9816511
ATACCAC454.3484564E-521.05010225
GATACCA552.029842E-417.2228124
TGATACC603.9422253E-415.78757723
ATGGGAG550.004143791714.7172645
TTGATAC1253.5923222E-712.9909227
AATGGGC956.734674E-512.7807834
ATCAACG1759.458745E-1012.334472
TCAACGC1852.2719178E-911.6677423
TATCAAC1751.2731107E-811.5635651
CAACGCA2151.6734703E-1011.2946444
GTTGATA1904.7932554E-79.97110227
GTATCAA1701.5009426E-59.5229361
CGTTGAT2801.538865E-99.182569527
GTGAATG7000.08.86541
TGAATGG6850.08.8625862
TTAGACG2359.473879E-78.6111654
GAATGGG7550.08.2195763
Produced by FastQC (version 0.11.2)