FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_45.3520000005dc1f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_45.3520000005dc1f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20227
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA730.36090374252237106No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT570.2818015523804815No Hit
GTGAATGGGGTATCTCGACGCTAAAAAAAAAAA440.21753102289019627No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA440.21753102289019627No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA430.21258713600632817No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA400.19775547535472388No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA360.17797992781925148No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA330.16314826716764722No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA310.15326049339991102No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA300.14831660651604292No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA300.14831660651604292No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA290.14337271963217482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.13842883274830672No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA280.13842883274830672No Hit
TATCAACGCAGAGTGAATGGGTAGCGTCAAAAA250.12359717209670243No Hit
GTGAATGGGTAGCGTCGAAAAAAAAAAAAAAAA240.11865328521283433No Hit
ACGCAGAGTGAATGGGTAGCGTCAAAAAAAAAA230.11370939832896625No Hit
GTGAATGGGTAGCGTCGAGATAAAAAAAAAAAA220.10876551144509813No Hit
GTACGTATCTCGACGCTAAAAAAAAAAAAAAAA210.10382162456123005No Hit
ACGCAGAGTGAATGGGATAGCGTCAAAAAAAAA210.10382162456123005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCG500.002149184716.197037
TGGGGTA550.00405745114.7245726
ATGGGGT657.211968E-414.5357955
TGCGTTG1101.2009477E-613.49752527
TCTGCGT3200.013.49752527
TATCAAC1459.9717E-913.0969291
TCAACGC1501.7214916E-812.597693
ATCAACG1501.7214916E-812.597692
GTATCAA1358.319712E-712.057491
AATGGGG1605.570273E-710.9667394
CAACGCA1751.470944E-710.7980194
CTGCGTT1606.8186637E-610.12314426
CTCTGCG4450.09.70608526
AACGCAG2009.2260234E-79.4482675
TACTCTG4700.09.18980424
GTACTCT4900.09.0901723
GATACGT4950.08.9983518
ACTCTGC4800.08.9983525
AGATACG4950.08.9983517
ATACGTA5000.08.90836619
Produced by FastQC (version 0.11.2)