FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_44.3520000005dbf4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_44.3520000005dbf4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28934
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA1890.6532107555125458No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1180.4078247044998963No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA980.33870187322872747No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA950.32833344853805213No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA830.28685974977535084No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA800.2764913250846755No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.22464920163129884No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA590.20391235224994814No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC580.20045621068638972No Hit
ACGCAGAGTGAATGGGCAGAGTGGAAAAAAAAA570.19700006912283127No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA550.19008778599571438No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGAAAA460.1589825119236884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1589825119236884No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1589825119236884No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA440.1520702287965715No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA420.1451579456694546No Hit
CCCCAGAGTGGCTAAACGTACTCTGCGTTGATA390.13478952097877928No Hit
ATCAACGCAGAGTGAATGGGCAGAGTGGCAAAA380.13133337941522086No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA380.13133337941522086No Hit
GTGAATGGGACAGAGTGGCAAAAAAAAAAAAAA380.13133337941522086No Hit
GTATCAACGCAGAGTGAATGGGCAGAGTGGCAA370.1278772378516624No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA370.1278772378516624No Hit
GTGAATGGGGCAGAGTGGAAAAAAAAAAAAAAA370.1278772378516624No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA370.1278772378516624No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.12442109628810397No Hit
AACGCAGAGTGAATGGGCAGAGTGGAAAAAAAA360.12442109628810397No Hit
ACGCAGAGTGAATGGGCAGAGTGAAAAAAAAAA300.10368424690675329No Hit
GTGAATGGGCAGAGTGAAAAAAAAAAAAAAAAA290.10022810534319486No Hit
GAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.10022810534319486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA257.6780975E-426.9792391
AGCCAGA351.9832782E-423.1250616
GTTGATA603.248897E-822.56076427
GTATCAA1200.021.3585641
TCTGCGT7700.015.11864227
TATCAAC2500.014.56878951
CGTTGAT1202.1466076E-713.53645827
ATCAACG2850.012.3063192
CTCTGCG10100.011.526093526
GCCAGAG1351.2476359E-510.9915427
TCAACGC2653.6379788E-1210.6898873
ACTCTGC11050.010.53516325
GTACTCT11350.010.37596323
TACTCTG11250.010.3478724
CAACGCA2655.2750693E-1110.1808454
GCGTTGA1751.8025967E-610.05565526
AGTGGCT11500.09.97058812
CGTACTC12300.09.90472622
ACGTACT12550.09.88880521
GTGGCTA11650.09.84221213
Produced by FastQC (version 0.11.2)