FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_43.3520000005dbe7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_43.3520000005dbe7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35113
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC5411.5407398969042805No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA1760.5012388574032409No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA1420.4044086235867058No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA960.27340301312904053No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT930.264859168968758No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA890.253467376755048No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA810.23068379232762795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.22213994816734545No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA770.21929200011391792No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA720.20505225984678038No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.18226867541936034No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA610.17372483125907784No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA610.17372483125907784No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA570.1623330390453678No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA550.1566371429385128No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA540.1537891948850853No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA500.14239740267137527No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG500.14239740267137527No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA490.13954945461794777No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA480.13670150656452026No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA470.13385355851109276No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.13385355851109276No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT460.13100561045766523No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA390.11106997408367272No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA370.10537407797681769No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA360.10252612992339019No Hit
ACGCAGAGTGAATGGGACATCCAGAAAAAAAAA360.10252612992339019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC902.9831426E-1019.53817627
TATCAAC2450.017.0663131
TGATACC1251.4133548E-915.149625
TTGATAC1251.4133548E-915.149624
ACACATC1004.3854925E-714.836687
TCAACGC2650.014.7603343
ATCAACG2650.014.7603342
GATACCA1252.3483153E-814.06748626
CAACGCA2800.013.9696024
CACACAT700.001303752213.4878916
GTTGATA1501.839544E-812.62466723
TCTGCGT7250.011.94057127
AACGCAG3050.011.9401015
TCACATC1154.0115474E-410.5557417
CTCTGCG9150.09.60893826
ATGGGAC2001.0210406E-69.44152455
TGGGACA1905.523898E-69.2285586
CGTTGAT2101.9075615E-69.01761822
ACTCTGC9950.08.83636125
ACGCAGA4250.08.5687786
Produced by FastQC (version 0.11.2)