FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_38.3520000005db3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_38.3520000005db3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20529
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT4482.182278727653563No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2981.4516050465195578No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA2111.0278143114618343No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC2101.022943153587608No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA2020.9839738905937941No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA1930.9401334697257538No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1690.823225680744313No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1490.7258025232597789No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA1460.7111890496370987No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC1360.6624774708948317No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1280.623508207901018No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA1230.5991524185298845No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1220.5942812606556579No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC1200.5845389449072045No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA1200.5845389449072045No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA1180.5747966291587511No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA1100.5358273661649374No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA1090.5309562082907107No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA990.48224462954844366No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG960.46763115592576354No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA930.4530176823030835No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA920.4481465244288567No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA920.4481465244288567No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA910.44327536655463007No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA850.4140484193092698No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC800.3896926299381363No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA750.3653368405670028No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA680.33123873544741583No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA680.33123873544741583No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG610.29714063032782895No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA580.28252715670514883No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA550.2679136830824687No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA540.263042525208242No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA520.2533002094597886No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA520.2533002094597886No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA510.2484290515855619No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA510.2484290515855619No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA500.24355789371133515No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA500.24355789371133515No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC500.24355789371133515No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA490.2386867358371085No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA470.22894442008865507No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA470.22894442008865507No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA440.21433094646597498No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA440.21433094646597498No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC440.21433094646597498No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA430.20945978859174827No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA430.20945978859174827No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA420.20458863071752156No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA420.20458863071752156No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA410.19971747284329486No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA410.19971747284329486No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT400.19484631496906815No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA400.19484631496906815No Hit
GTGAATGGGCAACAACGTACTCTGCGTAAAAAA400.19484631496906815No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA390.18997515709484145No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA380.18510399922061474No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.18510399922061474No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA380.18510399922061474No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.18023284134638803No Hit
CAACGTACTCTAAAAAAAAAAAAAAAAAAAAAA350.17049052559793462No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA340.16561936772370792No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA330.16074820984948124No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA320.1558770519752545No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA320.1558770519752545No Hit
GCAACAACGTACTCTAAAAAAAAAAAAAAAAAA310.1510058941010278No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA310.1510058941010278No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA300.14613473622680112No Hit
TCAACGCAGAGTGAATGGGCAACAACGTACTCT290.14126357835257441No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA280.13639242047834768No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.131521262604121No Hit
GTACGTTGTTGCCAAAAAAAAAAAAAAAAAAAA270.131521262604121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1266501047298943No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA250.12177894685566758No Hit
GAGTACGTTGTTGCCAAAAAAAAAAAAAAAAAA250.12177894685566758No Hit
ACGTACTCAAAAAAAAAAAAAAAAAAAAAAAAA250.12177894685566758No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA240.11690778898144089No Hit
CAACAACGTACTCTGCGTAAAAAAAAAAAAAAA240.11690778898144089No Hit
ATGTTCGGCAACAACGTACTCTGCGTTGATACC240.11690778898144089No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA230.11203663110721418No Hit
GGGCAACAACGTACTCAAAAAAAAAAAAAAAAA230.11203663110721418No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA230.11203663110721418No Hit
AACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA230.11203663110721418No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10716547323298749No Hit
GTTGTTGCCAAAAAAAAAAAAAAAAAAAAAAAA220.10716547323298749No Hit
GCAACAACGTACTCTGCGAAAAAAAAAAAAAAA210.10229431535876078No Hit
TTCGGCAACAACGTACTCTGCGTTGATACCACT210.10229431535876078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGTT200.00846606226.9682186
TTATGTT200.00846606226.9682184
GCTGTTC657.2720635E-414.5213467
TATCAAC1703.45608E-1113.5171581
TCTGCGT3950.012.34970327
GTATCAA1401.3507106E-611.5861341
TGCGTTG1351.1391645E-511.04104127
TGCGAAA1102.5626883E-411.03245212
TCTGCGA1102.5626883E-411.03245210
CTGCGAA1102.5626883E-411.03245211
AATGGGT900.00836542610.4876394
GCGAAAA1050.002195895210.27360613
TCAACGC2101.4988473E-810.2736063
ATCAACG2152.1624146E-810.0346842
GTGTTCG1351.489213E-49.9882289
AATGGGG1100.00323108759.8066234
CGAAAAA1100.00323108759.80662314
CTGTTCG2902.6193447E-109.2993858
CGGCAAC5800.08.83441513
CAACGCA2502.2210224E-78.6298294
Produced by FastQC (version 0.11.2)