FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_35.3520000005dae8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_35.3520000005dae8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67343
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC9121.3542610219324949No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3960.5880343911022675No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.2361047176395468No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1290.19155665770755684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.1900717223764905No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC1070.15888808042409752No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1000.1484935331066332No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA930.13809898578916888No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA900.13364417979596988No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA850.12621950314063823No Hit
GAGTACGTCACAAAAAAAAAAAAAAAAAAAAAA820.12176469714743922No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT820.12176469714743922No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA820.12176469714743922No Hit
GTGAATGGGAGTCCTAAAAAAAAAAAAAAAAAA810.1202797618163729No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA780.1158249558231739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT405.94684E-726.995541
TATCTAC257.758524E-426.995541
TATCATC405.94684E-726.995542
CAACTCA501.5212572E-724.2959881
GATACCA1450.024.22089626
TCGCAGA451.6607919E-623.9960387
ATCGCAG451.6607919E-623.9960386
TCATCGC451.6607919E-623.9960384
ATACCAC1500.023.4135327
TCTACGC300.002244850222.4962843
TGATACC1600.021.95018625
ATAAGTC504.147576E-621.5964346
ATCATCG504.147576E-621.5964343
CATCGCA504.147576E-621.5964345
TTGATAC1700.021.45357524
GTTGATA1800.020.26171123
TACGCAG404.965722E-420.2466565
ATCTACG350.005485269219.282532
CTACGCA350.005485269219.282534
TCAGAGT753.708683E-717.9970285
Produced by FastQC (version 0.11.2)