FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_34.3520000005dab1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_34.3520000005dab1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20827
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2561.2291736687953139No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1270.6097853747539252No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA970.4657415854419744No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA830.3985211504297306No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA810.38891823114226726No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA750.3601094732798771No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA740.3553080136361454No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA730.3505065539924137No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA700.33610217506121864No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA690.3313007154174869No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA600.28808757862390166No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA600.28808757862390166No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA590.28328611898017No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC590.28328611898017No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT590.28328611898017No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA540.25927882076151154No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA540.25927882076151154No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA530.2544773611177798No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA500.24007298218658474No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA500.24007298218658474No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA480.23047006289912136No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA480.23047006289912136No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA470.22566860325538965No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA420.20166130503673113No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA410.19685984539299944No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.19685984539299944No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA400.19205838574926778No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG400.19205838574926778No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA390.1872569261055361No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.18245546646180438No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA380.18245546646180438No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA370.1776540068180727No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA330.15844816824314592No Hit
CCATCGAGACGAGTACGTACTCTGCGTTGATAC320.15364670859941423No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC300.14404378931195083No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGT300.14404378931195083No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC300.14404378931195083No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA290.13924232966821914No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT280.13444087002448746No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA280.13444087002448746No Hit
GTGAATGGGGATCGAGACAAAAAAAAAAAAAAA260.12483795073702407No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT260.12483795073702407No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA260.12483795073702407No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA260.12483795073702407No Hit
GACGAGTACGTACAAAAAAAAAAAAAAAAAAAA250.12003649109329237No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACAAA240.11523503144956068No Hit
GAATGGGATCGAGACAAAAAAAAAAAAAAAAAA240.11523503144956068No Hit
AACGCAGAGTGAATGGGATCGAGACGAGAAAAA230.11043357180582898No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA230.11043357180582898No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA220.1056321121620973No Hit
GTGAATGGGATCGAGACGAGTAAAAAAAAAAAA220.1056321121620973No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10083065251836557No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA210.10083065251836557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1650.016.3636361
TATCAAC2350.014.3617031
TCAACGC2650.012.2264163
ATCAACG2650.012.2264162
CAACGCA2550.012.176474
AACGCAG3151.8189894E-129.8571425
GCGTTGA1555.4748627E-59.58064627
TCTGCGT3703.6379788E-128.75675727
TGCGTTG2203.38483E-68.59090927
ACGCAGA4550.08.3076931
TGGGATC1700.00147878977.9411776
GCAGAGT4301.2732926E-117.84883743
ATGGGAT1904.9998553E-47.81578975
CAGAGTG4503.6379788E-127.79999974
CGCAGAG4503.6379788E-127.79999972
TATCGAG2101.6824385E-47.71428545
CTGCGTT2501.8850737E-57.5626
AGAGTGA4659.094947E-127.54838665
CTCTGCG4301.07320375E-107.534883526
ACTCTGC4707.585186E-106.89361725
Produced by FastQC (version 0.11.2)