FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_30.3520000005da48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_30.3520000005da48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26890
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA2450.9111193752324285No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA1130.4202305689847527No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC980.36444775009297137No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA870.32354034957233174No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC830.30866493120119004No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT830.30866493120119004No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA780.2900706582372629No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA630.2342878393454816No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA610.22685013015991073No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA590.21941242097433988No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA580.21569356638155446No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA570.21197471178876903No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA560.20825585719598363No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA490.18222387504648568No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT470.17478616586091483No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA450.167348456675344No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA420.1561918928969877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.14875418371141688No Hit
GCTAAATACGGGCGAGAGACCGATAGCGAACAA390.14503532911863146No Hit
GTTTGGGAATGCAGCCCCAATCGGGCGGTAAAT360.1338787653402752No Hit
GCCCCAATCGGGCGGTAAATTCCGTCCAAGGCT360.1338787653402752No Hit
TTCGTCACACTACGTACTCTGCGTTGATACCAC350.13015991074748978No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA340.12644105615470436No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA290.10784678319077723No Hit
CTGCAAAACCTAGGGCGCGAGCCCGGGCGGAGC290.10784678319077723No Hit
GGCCTAAGTTCCCTGGAAAGGGGCGCCAGAGAG280.10412792859799182No Hit
GCGCTTAAGCGCGCGACCTATACCCGGCCGTCG280.10412792859799182No Hit
AACGCAGAGTGAATGGGTTCGTCAAAAAAAAAA280.10412792859799182No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA270.10040907400520639No Hit
GTCCAAGGCTAAATACGGGCGAGAGACCGATAG270.10040907400520639No Hit
TGCCCGGACCCTGTCGCACCACGAGGCGCTGTC270.10040907400520639No Hit
GCATTGCGATGGTCCCTGCGGATGCTAACGCAA270.10040907400520639No Hit
TATCAACGCAGAGTGAATGGGTTCGTCAAAAAA270.10040907400520639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTCG300.002207947422.5000027
TGATACC404.8539485E-420.2526
GATACCA450.001072324818.027
TCTGCGT3850.015.42857327
TTGATAC600.00730174913.50000125
CGGTTCG600.00730174913.5000017
TATCAAC1401.028111E-712.5357141
GCGTTGA1051.6824642E-411.57142827
CTCTGCG5200.011.42307726
TCAACGC1553.865007E-711.322583
ATCAACG1553.865007E-711.322582
TGTTCGT1452.1779724E-611.1724148
ATGGGGT1000.001474021210.7999995
CAACGCA1751.8481605E-610.0285724
ACTCTGC6100.09.95901625
TGCGTTG1258.434377E-49.71999926
GGTTCGT2502.1736923E-99.7199998
GTATCAA1555.640406E-59.5806451
TACTCTG6400.09.2812524
GTACTCT6450.09.20930223
Produced by FastQC (version 0.11.2)