FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_3.3520000005d6df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_3.3520000005d6df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61399
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA11611.8909102754116516No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC3320.5407254189807652No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1610.26221925438525057No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1150.18729946741803613No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1090.1775273212918777No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1020.16612648414469292No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA940.1530969559764817No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA880.14332480985032328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.1302952816821121No Hit
ACTCTGTTCCTCACGTACTCTTCGTTGATACCA780.1270378996400593No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA750.1221518265769801No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA730.11889444453492728No Hit
CCACTCTGTTCCTCACGTACTCTGCGTTGATAC640.10423622534568967No Hit
GTGAATGGGACTCTGTTCAAAAAAAAAAAAAAA620.10097884330363686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATA200.00857321826.9836624
TGGGAGA352.0167758E-423.128856
ATACCAC300.00222697522.52318627
GGGAGAG300.002248281122.4863837
AGCACTC559.475158E-619.6244816
GGAGAGT350.00549365119.2740428
CCCACAC350.00549365119.2740422
AACACTC501.0192597E-418.8885616
GATACCA956.566552E-1018.49272227
TGATACC956.566552E-1018.49272226
GCGTTGA5050.016.05613327
CGGACTC550.004197221214.718366
ACCACTC657.540261E-414.5296646
ATGGGAG751.3539314E-414.3912875
TATCAAC2350.012.6409771
TTGATAC1554.0923805E-711.34353225
ATCAACG2501.8189894E-1211.33313752
GCACTCT1701.1492739E-711.1109197
TCTGCGT16650.010.95722527
TGCGTTG8250.010.64732527
Produced by FastQC (version 0.11.2)