FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_27.3520000005d9e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_27.3520000005d9e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16206
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA860.5306676539553251No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT780.4813032210292484No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA690.42576823398741204No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA610.3764038010613353No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA520.32086881401949896No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA500.3085277057879798No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA500.3085277057879798No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA470.290016043440701No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA470.290016043440701No Hit
ACGCAGAGTGAATGGGGTACGTTTCGACCAAAA420.259163272861903No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA390.2406516105146242No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA390.2406516105146242No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA380.23448105639886463No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA370.228310502283105No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA340.20979883993582624No Hit
GTACGTTTCGACCAAAAAAAAAAAAAAAAAAAA330.20362828582006662No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA330.20362828582006662No Hit
GTGAATGGGGAGTACGTTTCGACCAAAAAAAAA330.20362828582006662No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA320.19745773170430705No Hit
ACGCAGAGTGAATGGGTCGTTGGAAAAAAAAAA310.19128717758854746No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC290.17894606935702828No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.16660496112550907No Hit
GAGTGAATGGGGTACGTTTCGACCAAAAAAAAA270.16660496112550907No Hit
GTGAATGGGTACGTTTCGACCAAAAAAAAAAAA240.1480932987782303No Hit
GGTACGTTTCGACCAAAAAAAAAAAAAAAAAAA240.1480932987782303No Hit
ACGCAGAGTGAATGGGTCGTTGGTCAAAAAAAA230.1419227446624707No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA210.1295816364309515No Hit
TATCAACGCAGAGTGAATGGGTCGTTGGTCAAA210.1295816364309515No Hit
GAGTGAATGGGGTCGTTGGAAAAAAAAAAAAAA210.1295816364309515No Hit
GAGTGAATGGGATCGTTGGAAAAAAAAAAAAAA210.1295816364309515No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT200.1234110823151919No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA200.1234110823151919No Hit
GAGTGAATGGGATCGTTGGTCAAAAAAAAAAAA200.1234110823151919No Hit
GTCGTTGGTCGAAACGTACTCTGCGTTGATACC200.1234110823151919No Hit
GTGAATGGGAGTACGTTTCGACCAAAAAAAAAA190.11724052819943231No Hit
GTGAATGGGGTCGTTGGAAAAAAAAAAAAAAAA180.11106997408367272No Hit
GTGAATGGGATCGTTGGTCAAAAAAAAAAAAAA180.11106997408367272No Hit
ACGCAGAGTGAATGGGGTCGTTGGAAAAAAAAA170.10489941996791312No Hit
ACGCAGAGTGAATGGGGAGTACGTTTCGACCAA170.10489941996791312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1053.9697625E-815.4162251
TCTGCGT3800.013.17488327
ATCAACG1305.4068187E-712.4515672
TGCGTTG1553.4046752E-711.34854627
TCAACGC1452.0253647E-611.1634733
AATGGGT1000.001429059710.7913584
CTCTGCG4750.010.53990626
ACTCTGC5050.09.91377325
TTCGTTG1555.318573E-59.5729798
CTCGTTG2305.686161E-89.383798
TACTCTG5400.09.27121324
GTGAATG5400.09.2425991
GTACTCT5500.09.10264623
TGAATGG5200.09.07926752
CTGCGTT1956.508644E-69.02063926
CGTACTC5550.09.02063922
ACGTACT5600.08.94009921
AACGTAC5650.08.83363420
GAAACGT5700.08.51949318
CGAAACG5700.08.51949317
Produced by FastQC (version 0.11.2)