FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_26.3520000005d9cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_26.3520000005d9cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42135
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA7851.8630592144298088No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA5661.3433012934614927No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5221.238875044499822No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA4170.9896760412958348No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA4160.9873027174557968No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT3680.8733831731339742No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA3200.7594636288121515No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA2830.6716506467307464No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA2590.6146908745698351No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA2440.5790910169692655No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA2430.5767176931292275No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA2400.5695977216091136No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA2060.4889047110478224No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA1890.4485582057671769No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA1860.4414382342470631No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1860.4414382342470631No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA1840.4366915865669871No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1650.3915984336062656No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA1590.37735849056603776No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA1570.3726118428859618No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA1470.34887860448558206No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1460.3465052806455441No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1410.3346386614453542No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA1330.3156520707250504No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA1320.31327874688501245No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA1310.3109054230449745No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA1270.3014121276848226No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA1220.28954550848463273No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA1200.2847988608045568No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA1200.2847988608045568No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA1120.265812270084253No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA1020.24207903168387326No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA1020.24207903168387326No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA1010.23970570784383527No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA990.23495906016375934No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.2325857363237214No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.2278390886436454No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA940.22309244096356948No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA930.22071911712353154No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA880.20885249792334165No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA880.20885249792334165No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA870.20647917408330366No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA860.2041058502432657No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.20173252640322772No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA840.19935920256318973No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT830.1969858787231518No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA820.1946125548831138No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA800.18986590720303786No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC770.18274593568292394No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA760.18037261184288597No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC760.18037261184288597No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.177999288002848No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA750.177999288002848No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA740.17562596416281No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA710.1685059926426961No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1685059926426961No Hit
TATCAACGCAGAGTGAATGGGACACAGAAAAAA700.16613266880265815No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA700.16613266880265815No Hit
ACGCAGAGTGAATGGGACACAGAAAAAAAAAAA680.16138602112258219No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA660.15663937344250622No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA660.15663937344250622No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA640.15189272576243026No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG640.15189272576243026No Hit
CAACGCAGAGTGAATGGGACACAGACAAAAAAA620.14714607808235433No Hit
ACGCAGAGTGAATGGGACACAAAAAAAAAAAAA580.13765278272220244No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA580.13765278272220244No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA570.13527945888216447No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC560.1329061350421265No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA560.1329061350421265No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC550.13053281120208854No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGTAC550.13053281120208854No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA550.13053281120208854No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA540.12815948736205054No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA530.12578616352201258No Hit
GAGTGAATGGGACACAGAAAAAAAAAAAAAAAA530.12578616352201258No Hit
CAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA520.1234128396819746No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA520.1234128396819746No Hit
GAGTAAATGGGACACAGACAAAAAAAAAAAAAA510.12103951584193663No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA510.12103951584193663No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA510.12103951584193663No Hit
ACGCAGAGTGAATGGGGACACAGACAAAAAAAA490.1162928681618607No Hit
GAATGGGACACAGAAAAAAAAAAAAAAAAAAAA470.11154622048178474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.11154622048178474No Hit
GAATGGGACACAGACAGTACAAAAAAAAAAAAA460.10917289664174677No Hit
AACGTAGAGTGAATGGGACACAGACAAAAAAAA460.10917289664174677No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT460.10917289664174677No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAAA460.10917289664174677No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10205292512163285No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAA430.10205292512163285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGG200.00852901126.9928673
GTGTATG200.00852901126.9928671
CACGACA200.00852901126.9928676
CAACTCA900.025.4932611
ATCTACG501.4944817E-724.293582
TCTACGC501.4944817E-724.293583
TATCTAC501.4944817E-724.293581
CTACGCA501.4944817E-724.293584
TACGCAG451.6375325E-623.9936585
GAGTAAA401.8028946E-523.618761
AGTAAAT401.8028946E-523.618762
TATCATC351.9975485E-423.1367442
AACTCAG1050.021.8513662
TCAGAGT951.8189894E-1221.3101565
TAAATGG454.4778622E-520.9944524
ACTCAGA1100.020.8581243
CTCAGAG1050.020.5659924
GTATCAT404.9264095E-420.244651
GTAAATG404.9264095E-420.244653
GAATGGT350.005454846719.28061910
Produced by FastQC (version 0.11.2)