FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_24.3520000005d98c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_24.3520000005d98c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34153
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC4331.2678242028518725No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC4051.1858401897344304No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1710.5006880801100928No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1550.4538400726144116No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1480.4333440693350511No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1300.38064006090240976No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1190.34843205574912894No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1170.3425760548121688No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1050.3074400491904079No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA980.2869440459110474No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA960.28108804497408724No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA910.26644804263168687No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA890.2605920416947267No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC880.2576640412262466No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA770.22545603607296577No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC730.21374403419904547No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC700.20496003279360525No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA670.19617603138816503No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT660.19324803091968495No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA650.19032003045120488No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC650.19032003045120488No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA590.17275202764032443No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC570.1668960267033643No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA560.1639680262348842No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA540.15811202529792406No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA540.15811202529792406No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC520.15225602436096392No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA510.14932802389248384No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT510.14932802389248384No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA500.14640002342400377No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA490.1434720229555237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.14054402248704362No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA470.13761602201856354No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC450.1317600210816034No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC440.1288320206131233No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC440.1288320206131233No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA390.11419201827072292No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA370.10833601733376277No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA360.1054080168652827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC752.5465852E-1123.42418127
GATACCA806.184564E-1121.96016926
TTGATAC2900.016.30993827
GTTGATA3050.015.50781126
TGATACC1157.888957E-915.27663925
CGTTGAT3500.013.5139525
ATGGGGG600.00737667313.4941355
TATCAAC1655.2132236E-912.2854091
TGGGGTG1255.3566455E-611.8748396
GGGGTGT1255.3566455E-611.8748397
ACCTCCA800.003540410211.8073686
GGTGTGT1051.7153608E-411.5664019
GCGTTGA4000.011.48685724
CGTCCAC1102.642711E-411.0406567
ATGGGGT1852.9021976E-810.941195
GGGTGTG1257.0879716E-510.7953088
TCAACGC1802.3832945E-710.4954383
ATCAACG1802.3832945E-710.4954382
TCTGCGT9500.010.38440427
GTATCAA1607.1797313E-610.1354631
Produced by FastQC (version 0.11.2)