FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_22.3520000005d93c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_22.3520000005d93c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46217
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC5281.1424367656922778No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA5091.1013263517753207No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA4080.8827920462167601No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA2990.6469480926931648No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA2700.5842006188199147No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC2480.5365990869160698No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA2450.5301079689291819No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA2440.5279442629335526No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA2340.5063072029772594No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC2260.4889975550122249No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA2090.4522145530865266No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA1640.3548477832832075No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA1640.3548477832832075No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA1460.31590107536187984No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA1340.2899366034143281No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA1210.26180842547114697No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA1130.24449877750611246No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC1130.24449877750611246No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA1120.24233507151048317No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA1080.23368024752796593No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA1050.22718912954107795No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA1040.22502542354544863No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA1020.22069801155419003No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC930.20122465759352617No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC920.19906095159789688No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC910.19689724560226757No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA900.19473353960663825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.19256983361100893No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA870.1882424216197503No Hit
GAGTGAATGGGGTAGTTCAAAAAAAAAAAAAAA870.1882424216197503No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA800.1730964796503451No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA790.1709327736547158No Hit
ACGCAGAGTGAATGGGTAGTTCAAAAAAAAAAA780.16876906765908647No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACAAA780.16876906765908647No Hit
TATCAACGCAGAGTGAATGGGTAGTTCAAAAAA780.16876906765908647No Hit
AACGCAGAGTGAATGGGGTAGTTCAAAAAAAAA760.16444165566782784No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA740.16011424367656923No Hit
GAATGGGGTAGTTCAAAAAAAAAAAAAAAAAAA710.15362312568968128No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT700.15145941969405197No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC700.15145941969405197No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC690.14929571369842265No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA680.14713200770279333No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA670.14496830170716404No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.14496830170716404No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA650.1406408897159054No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA640.1384771837202761No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA630.13631347772464678No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG620.13414977172901746No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA620.13414977172901746No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA590.1276586537421295No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA590.1276586537421295No Hit
GGGTAGTTCAAAAAAAAAAAAAAAAAAAAAAAA590.1276586537421295No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA580.1254949477465002No Hit
AACGCAGAGTGAATGGGGTAGTTCTGACACGTA560.12116753575524158No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA550.11900382975961227No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.11467641776835363No Hit
GTAGTTCTGACACGTACTCTGCGTTGATACCAC530.11467641776835363No Hit
ACGCAGAGTGAATGGGTAGTTCTGACAAAAAAA510.11034900577709501No Hit
TGGTAGTTCTGACACGTACTCTGCGTTGATACC500.1081852997814657No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA470.10169418179457775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT653.6379788E-1227.0000021
TATCATC653.6379788E-1227.0000022
TCTGCGG200.00852641727.027
CAACTCA950.025.5789471
TCGCAGA701.0913936E-1125.0714287
ATCGCAG701.0913936E-1125.0714286
CATCGCA701.0913936E-1125.0714285
ATCATCG651.1641532E-1024.9230793
TCATCGC651.1641532E-1024.9230794
TATCTAC950.022.7368431
AACGTAG300.002232048422.51
TGATACC756.9849193E-1021.627
ATCTACG1000.021.5999982
TCTACGC1000.021.5999983
CTACGCA1000.021.5999984
AACTCAG1150.021.1304362
ACTCAGA1150.021.1304363
CTCAGAG1100.020.8636364
TACGCAG1050.020.571435
TCAGAGT1050.020.571435
Produced by FastQC (version 0.11.2)