FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_21.3520000005d92f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_21.3520000005d92f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32771
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA1430.4363614171065881No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1060.3234567147783101No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA1040.3173537578957005No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1000.3051478441304812No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA870.26547862439351866No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT840.2563241890696042No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA770.23496383998047052No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA690.21055201245003202No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA670.2044490555674224No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA620.18919166336089838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.18003722803698394No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.18003722803698394No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA570.1739342711543743No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA560.1708827927130695No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA500.1525739220652406No Hit
ACCCAGTTCAAGGTACGTACTCTGCGTTGATAC490.1495224436239358No Hit
TATCAACGCAGAGTGAATGGGCCAGTTCAAGGT470.14341948674132615No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA470.14341948674132615No Hit
GCCCAGTTCAAGGTACGTACTCTGCGTTGATAC460.14036800830002136No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA440.13426505141741174No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT420.1281620945348021No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA420.1281620945348021No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG400.12205913765219248No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA400.12205913765219248No Hit
ACGCAGAGTGAATGGGCCAGTTCAAGGTAAAAA390.11900765921088767No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA360.10985322388697323No Hit
TCCCAGTTCAAGGTACGTACTCTGCGTTGATAC360.10985322388697323No Hit
GTATCAACGCAGAGTGAATGGGCCAGTTCAAGG360.10985322388697323No Hit
GTGAATGGGCCAGTTCAAGGAAAAAAAAAAAAA340.1037502670043636No Hit
GCCATGGGGAGATACCGTCTGTGATCCATGGAT330.10069878856305879No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA330.10069878856305879No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCAG200.00850500226.990846
GTAGCGG200.00850500226.990846
ACACCAG351.9854038E-423.1350086
GAGCCAG300.002222701422.4923676
TCTGCGT9700.018.25364127
TGGGCCC450.001081702217.99389518
GGGCCCA500.00219050416.19450419
TGCCAGT604.0254788E-415.7446577
TATCAAC2450.014.8725031
ATCCAGT657.393529E-414.5335297
CTCTGCG12800.013.83283826
GTACTCA600.00736378513.4954212
TCCAGTT2350.013.2082828
AGCCAGT956.7138346E-512.7851347
CTCCAGT956.7138346E-512.7851347
ATCAACG2750.012.7593052
ACTCTGC13900.012.738152525
TTGATAC1305.870588E-712.47635827
ACCCAGT1203.37844E-612.3708027
TACTCTG14400.012.295855524
Produced by FastQC (version 0.11.2)