FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_20.3520000005d905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_20.3520000005d905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46574
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT5491.1787692704083823No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA5331.1444153390303602No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA3680.7901404216945077No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA3580.7686692145832439No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA2550.5475157813372268No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA2440.5238974535148366No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA2070.4444539872031606No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1960.42083565938077044No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA1940.4165414179585177No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA1660.35642203804697903No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA1540.33065658951346244No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA1530.32850946880233606No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA1470.31562674453557776No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC1420.30489114097994585No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA1210.25980160604629193No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA1130.24262464035728087No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.23403615751277534No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA1070.2297419160905226No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA1020.2190063125348907No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1010.2168591918237643No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA1010.2168591918237643No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA980.21041782969038522No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA980.21041782969038522No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA930.1996822261347533No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.19538798471250055No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA880.1889466225791214No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA850.18250526044574228No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC750.16103405333447846No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA710.15244557048997293No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA710.15244557048997293No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAAAAA670.14385708764546742No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.14385708764546742No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA670.14385708764546742No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA640.1374157255120883No Hit
CAACTCAGAGTGAATGGGACGTAGGAGACAAAA620.13312148408983554No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC620.13312148408983554No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA610.13097436337870916No Hit
ACGTAGGAGACTAAAAAAAAAAAAAAAAAAAAA570.12238588053420363No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA570.12238588053420363No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA560.12023875982307725No Hit
GTATCATCGCAGAGTGAATGGGACGTAGGAGAC520.11165027697857173No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGACAA520.11165027697857173No Hit
GGTATCAACGCAGAGTGAATGGGACGTAGGAGA510.10950315626744535No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA500.10735603555631897No Hit
TATCTACGCAGAGTGAATGGGACGTAGGAGACA500.10735603555631897No Hit
GTGAATGGGCACGTAGGAGAAAAAAAAAAAAAA500.10735603555631897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.10520891484519261No Hit
GAGTGAATGGGACGTAGGAAAAAAAAAAAAAAA490.10520891484519261No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGAAAA490.10520891484519261No Hit
GAATGGGGACGTAGGAGAAAAAAAAAAAAAAAA480.10306179413406623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.410766E-826.9935281
TATCATC455.410766E-826.9935282
GTATGGG405.883503E-726.9935263
TAAATGG257.725208E-426.9935264
GTGTATG405.883503E-726.9935261
GGTTTCA200.00853686526.9935261
TGTATGG405.883503E-726.9935262
TATGGGA257.725208E-426.9935264
CAACTCA900.023.9942471
TACCACT401.7926559E-523.64484427
GAGTAAA352.0015285E-423.137311
AGTAAAT352.0015285E-423.137312
TCTACGC950.022.7313923
TATCTAC950.022.7313921
CTACGCA900.022.4946084
GTAAATG300.002235342722.4946063
ATCTACG1050.021.8519022
TACGCAG1050.020.5664965
TCAGAGT953.092282E-1119.8899675
CGCTGAG701.7669299E-719.2810922
Produced by FastQC (version 0.11.2)