FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_17.3520000005d8a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_17.3520000005d8a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18187
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA1800.9897179303898388No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1050.5773354593940727No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC840.46186836751525817No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC830.45636993456864794No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA820.45087150162203776No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.38489030626271514No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA640.3518997085830538No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA610.3354044097432232No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC580.3189091109033925No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA570.31341067795678235No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA490.2694232143839006No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC480.26392478143729037No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA450.2474294825974597No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA440.2419310496508495No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC350.19244515313135757No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA270.14845768955847582No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC270.14845768955847582No Hit
GTCATACGCACGTTACGTACTCTGCGTTGATAC270.14845768955847582No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT250.1374608236652554No Hit
GTGAATGGGACATACGCAAAAAAAAAAAAAAAA240.13196239071864518No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA240.13196239071864518No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC230.12646395777203498No Hit
CCCCATACGCACGTTACGTACTCTGCGTTGATA220.12096552482542475No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10996865893220434No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10996865893220434No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA190.1044702259855941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1352.1827873E-1015.0399021
TCAACGC1454.0017767E-1114.8950313
ATCAACG1403.947207E-1014.462812
CAACGCA1551.1823431E-1013.9340614
ACATACG1001.0317113E-412.1487618
AACGCAG1902.9631337E-911.3672615
TCTGCGT3000.011.24885227
CAGAGTG2451.4862962E-89.3663919
CTCTGCG3650.09.24563126
GTATCAA1350.00160973519.0239411
AGAGTGA2451.547578E-78.81542710
CGCAGAG2451.547578E-78.8154277
ACGCAGA2502.1102824E-78.6391196
ACTCTGC3951.8189894E-128.54343225
GTACTCT4001.8189894E-128.4366423
CGTACTC4001.8189894E-128.4366422
TACTCTG4001.8189894E-128.4366424
ACGTACT4053.6379788E-128.33248321
GCAGAGT2603.8456528E-78.3068458
ACGTTAC4103.6379788E-128.23086716
Produced by FastQC (version 0.11.2)