FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_14.3520000005d83d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_14.3520000005d83d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33553
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA7472.226328495216523No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC5161.537865466575269No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA1960.5841504485440945No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA1760.524543259917146No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA1750.5215629004857986No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA1660.4947396656036718No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA1530.45599499299615537No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA1390.41426996095729146No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA1330.39638780436920695No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.36956456948708016No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA1160.34572169403630076No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA1100.32783953744821626No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA1080.3218788185855214No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.3069770214287843No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC990.29505558370339463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.2801537865466575No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC940.2801537865466575No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA920.2741930676839627No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA880.262271629958573No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA810.24140911393914105No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA780.2324680356450988No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA780.2324680356450988No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA770.22948767621375135No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA750.22352695735105652No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA730.21756623848836168No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA710.21160551962566684No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA710.21160551962566684No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA700.20862516019431945No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA700.20862516019431945No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT700.20862516019431945No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.205644800762972No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA660.19670372246892975No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT660.19670372246892975No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA660.19670372246892975No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA640.19074300360623492No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA620.18478228474354008No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA600.17882156588084525No Hit
TATCAACGCAGAGTGAATGGGCAACAGAAAAAA590.17584120644949783No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA590.17584120644949783No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA580.17286084701815038No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA570.16988048758680296No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT560.16690012815545555No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA540.1609394092927607No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA510.15199833099871846No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA490.14603761213602362No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.14305725270467617No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.14305725270467617No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCATAC470.14007689327332876No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA450.13411617441063392No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA450.13411617441063392No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCAAA430.12815545554793908No Hit
CAACGCAGAGTGAATGGGCAACAGAGCAAAAAA420.12517509611659167No Hit
ACGCAGAGTGAATGGGGCAACAGAAAAAAAAAA420.12517509611659167No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA420.12517509611659167No Hit
CAACTCAGAGTGAATGGGCAACAGAGAAAAAAA410.12219473668524425No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC410.12219473668524425No Hit
ACGCAACAGAGCATACGTACTCTGCGTTGATAC410.12219473668524425No Hit
GGGCAACAGAGAAAAAAAAAAAAAAAAAAAAAA400.11921437725389683No Hit
GGGCAACAGAGCATACGTACTCTGCGTTGATAC400.11921437725389683No Hit
GTGCAACAGAGCATACGTACTCTGCGTTGATAC400.11921437725389683No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.1162340178225494No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC380.11325365839120198No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT380.11325365839120198No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA370.11027329895985456No Hit
CAACAGAGAAAAAAAAAAAAAAAAAAAAAAAAA360.10729293952850714No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA360.10729293952850714No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.10431258009715973No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA350.10431258009715973No Hit
GAGTGAATGGGCAACAGAGCATAAAAAAAAAAA340.1013322206658123No Hit
GAGTGAATGGGCAACAGAAAAAAAAAAAAAAAA340.1013322206658123No Hit
GTATCAACGCAGAGTGAATGGGGCAACAGAGCA340.1013322206658123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA200.00847309527.01343311
AGAGCGA200.00847309527.01343310
TCGCAGA257.6471484E-427.0134327
ATCGCAG257.6471484E-427.0134326
TATCATC257.714324E-426.9731752
ATCATCG257.714324E-426.9731753
TCATCGC257.714324E-426.9731754
GCGAATG200.00853513926.97317313
CGAATGG200.00853513926.97317314
AGCGAAT200.00853513926.97317312
ATACCAC657.856215E-820.77956627
CATCGCA350.005408233519.295315
GTATCAT350.00545522919.2665521
TATCAAC3250.017.0138471
GTATCAA1500.016.1839051
ATCAACG3400.015.8665732
TCAACGC3600.015.3597253
CAACGCA4000.013.8237514
GATACCA1152.9949857E-511.7449726
AACGCAG4500.011.7058225
Produced by FastQC (version 0.11.2)