FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_13.3520000005d813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_13.3520000005d813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34692
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCATAGAGTCACGTACTCTGCGTTGATACC2980.8589876628617549No Hit
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA2610.7523348322379799No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC2280.6572120373573158No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1650.4756139744033206No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1640.4727314654675429No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA1520.4381413582382105No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA1310.3776086705868788No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA1300.3747261616511011No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC900.2594258042199931No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA870.25077827741265996No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA750.21618817018332756No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA700.20177562550443903No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC650.18736308082555056No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA610.17583304508243974No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA590.17006802721088435No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA530.15277297359621814No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA530.15277297359621814No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA530.15277297359621814No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA520.14989046466044045No Hit
GAGTGAATGGGCACCATAGAAAAAAAAAAAAAA510.14700795572466274No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA490.14124293785310735No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA470.13547791998155193No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA460.13259541104577424No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA450.12971290210999656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.12683039317421882No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT420.12106537530266344No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA420.12106537530266344No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA390.11241784849533035No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT380.10953533955955262No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA370.10665283062377492No Hit
GTGAATGGGGAGTACGTGACTCTAAAAAAAAAA360.10377032168799724No Hit
AACGCAGAGTGAATGGGCACCATAGAGAAAAAA360.10377032168799724No Hit
CCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA360.10377032168799724No Hit
ACACCATAGAGTCAAAAAAAAAAAAAAAAAAAA350.10088781275221952No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA350.10088781275221952No Hit
ACACCATAGAGAAAAAAAAAAAAAAAAAAAAAA350.10088781275221952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1201.8189894E-1218.05031427
ATGGGAG601.9814099E-517.9720615
TATCAAC1451.8189894E-1215.8488251
GGTATCA603.9820684E-415.7711341
TTGATAC1402.5465852E-1115.47169926
ACTCTAT1057.415165E-714.1618361
GTTGATA1602.1827873E-1013.53773625
CGTTGAT1602.1827873E-1013.53773624
ATCAACG1704.0017767E-1113.4985522
TCAACGC1801.09139364E-1012.730213
CTCTATG1401.06401785E-712.534372
CAACGCA1902.6921043E-1012.06024
GCGTTGA1954.962203E-911.10788523
AGAGTAA850.005616639311.100395
TCTGCGT7100.011.05899527
TCTATGG1606.142891E-710.9517253
TGCGTTG3150.010.74423527
GTATCAA900.00849815310.4988752
AATGGGA1701.3416011E-610.3075064
TCCACCA1050.002270692710.269757
Produced by FastQC (version 0.11.2)