FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate4_10.3520000005d7c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate4_10.3520000005d7c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23169
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA5352.309119944753766No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3181.3725236307134534No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA1900.8200612887910571No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT1690.7294229358194139No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1670.7207907117268765No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1190.5136173335059778No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1120.4834045491820968No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA1030.4445595407656783No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA1020.4402434287194096No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA970.41866286848806594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.2503344986835858No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT570.2460183866373171No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA560.2417022745910484No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA510.2201217143597048No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA500.21580560231343604No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA490.21148949026716735No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA480.2071733782208986No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA460.19854115412836115No Hit
GAGTGAATGGGATGCAGAGAAAAAAAAAAAAAA430.18559281798955501No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA430.18559281798955501No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT400.17264448185074885No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA400.17264448185074885No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA400.17264448185074885No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA380.1640122577582114No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA380.1640122577582114No Hit
ACGCAGAGTGAATGGGATGCAGAGACAAAAAAA370.15969614571194268No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA360.15538003366567396No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT350.15106392161940524No Hit
GGAGAGACGTACGTACTCAAAAAAAAAAAAAAA340.14674780957313652No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA330.1424316975268678No Hit
GTGAATGGGATGCAGAGAAAAAAAAAAAAAAAA330.1424316975268678No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.13811558548059907No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGAAAA320.13811558548059907No Hit
ATGCAGAGACGTACGTACTCTTCGTTGATACCA320.13811558548059907No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA320.13811558548059907No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG310.13379947343433035No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC310.13379947343433035No Hit
ACGCAGAGTGAATGGGATGCAGAGAAAAAAAAA300.12948336138806163No Hit
GAGTGAATGGGAGAGACGTACGTACTCAAAAAA290.1251672493417929No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA290.1251672493417929No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA270.11653502524925548No Hit
GACGTACGTACTCTAAAAAAAAAAAAAAAAAAA270.11653502524925548No Hit
TATCAACGCAGAGTGAATGGGAGACGTACGTAC250.10790280115671802No Hit
GTGAATGGGGATGCAGAAAAAAAAAAAAAAAAA240.1035866891104493No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA240.1035866891104493No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1035866891104493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACT306.865176E-527.027
ATACCAC351.9564002E-423.14285926
TATCAAC2200.019.0227261
TGATACC906.2518666E-918.00000226
TTGATAC906.2518666E-918.00000225
ATCAACG2350.017.808512
GATACCA951.2212695E-817.05263127
TCAACGC2550.016.4117663
ATGGGGT500.002159460716.25
GTATCAA902.3203993E-615.0000011
CAACGCA2900.014.8965524
GTTGATA1151.2810051E-714.08695724
AACGCAG3200.013.0781255
CGTTGAT1359.0137473E-712.023
ACGCAGA4350.09.6206896
GCGTTGA2009.460746E-79.45000127
CGCAGAG4300.09.4186057
TGCGTTG2902.746674E-98.84482827
GCAGAGT4250.08.576478
CAGAGTG4200.08.3571429
Produced by FastQC (version 0.11.2)