FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_92.3520000005d5fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_92.3520000005d5fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56721
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA1640.2891345357098782No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.28560850478658695No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA1240.21861391724405424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.20803582447418062No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA1100.19393170078101585No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC1050.18511662347278787No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1020.17982757708785105No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA810.14280425239329347No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA770.1357521905467111No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.1198850513919007No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1145960050069639No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCAGG306.9814436E-527.03359826
AAGAGCC306.9814436E-527.03359823
GCCAGGC306.9814436E-527.03359827
GGCAAGA257.719452E-427.00971820
ACCTATA307.023681E-527.0097161
CTATACC356.4645574E-626.985883
CACTCAG200.00856448626.985885
CCTATAC307.066136E-526.9858782
CGGGGCA257.7594654E-426.98587817
CCGTCGG257.7594654E-426.98587813
CAGAGTT307.066136E-526.98587810
GAGTTGC307.066136E-526.98587812
AGAGTTG307.066136E-526.98587811
TGCTAAA307.066136E-526.98587816
CGTCGGG257.7594654E-426.98587814
CGGCCGT307.066136E-526.98587810
TACCCGG307.066136E-526.9858786
GCCGTCG257.7594654E-426.98587812
ATACCCG307.066136E-526.9858785
GTTGCTA307.066136E-526.98587814
Produced by FastQC (version 0.11.2)