FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_91.3520000005d5d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_91.3520000005d5d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29771
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC3041.0211279433005274No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA1900.6382049645628296No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA1140.38292297873769776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.3762050317422996No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA970.32582042927681304No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG810.27207685331362735No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA780.2619999328205301No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA740.24856403882973366No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT660.22169225084814081No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA660.22169225084814081No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA650.2183332773504417No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA650.2183332773504417No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA620.2082563568573444No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA610.2048973833596453No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA560.1881025158711498No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA420.14107688690336231No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA420.14107688690336231No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA410.13771791340566322No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA400.13435893990796413No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA390.13099996641026504No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA380.12764099291256592No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA370.12428201941486683No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT370.12428201941486683No Hit
TATCAACGCAGAGTGAATGGGACATCCAGAAAA370.12428201941486683No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA350.11756407241946862No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA350.11756407241946862No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCTA340.11420509892176951No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA340.11420509892176951No Hit
GAATGGGACATCCAGCAAAAAAAAAAAAAAAAA300.10076920493097309No Hit
ACGCAGAGTGAATGGGACATCCAGAAAAAAAAA300.10076920493097309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGA200.00847860327.03
ATACCAC601.2169039E-924.7527
GATACCA653.074092E-922.84615526
TGATACC653.074092E-922.84615525
TTGATAC707.232302E-921.21428724
GTTGATA751.6008926E-819.823
TGACATC552.0594528E-417.1818187
TCTGCGT9350.014.14973327
GCCACAT853.7864313E-412.7058836
CGTTGAT1203.336043E-612.37522
GTATCAA2201.8189894E-1212.2727271
TATCAAC2800.012.0535721
CTCTGCG11350.011.65638726
ACTCTGC12300.010.86585425
TGCGTTG3300.010.63636327
CTCACAT900.008444563510.56
TCAACGC3000.010.3499993
ATCAACG3050.010.1803282
TACTCTG13150.010.16349824
GCGTTGA2008.873212E-810.12527
Produced by FastQC (version 0.11.2)