FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_9.3520000005cba7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_9.3520000005cba7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14346
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC1471.0246758678377248No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC620.4321762163669316No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA600.41823504809703055No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC580.4042938798271295No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA480.3345880384776244No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC470.3276174543426739No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA420.2927645336679214No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA350.2439704447232678No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC290.20214693991356475No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA280.19517635577861425No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA240.1672940192388122No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA230.1603234351038617No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA220.15335285096891121No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA210.1463822668339607No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA160.11152934615920815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11152934615920815No Hit
GTCTACGTACCGTAACGTACTCTGCGTTGATAC160.11152934615920815No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA160.11152934615920815No Hit
CTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA150.10455876202425764No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA150.10455876202425764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC800.025.312527
TTGATAC951.8189894E-1221.3157926
TATCAAC607.8442645E-720.251
TCAACGC558.622894E-619.6363643
ATCAACG601.8271094E-518.02
GTTGATA1151.8189894E-1117.60869625
CGTTGAT1151.8189894E-1117.60869624
CAACGCA653.6361424E-516.6153854
AACGCAG802.1394534E-413.55
GCGTTGA1501.027729E-913.49999923
AATGGGG1102.4335874E-411.0454554
TCTGCGT3700.010.94594627
CCCTACG1050.0021056410.2857148
CAGAGTG1205.4452673E-410.1259
ACGCAGA1100.00309929169.8181826
GCAGAGT1100.00309929169.8181828
CGCAGAG1100.00309929169.8181827
TGCGTTG1955.9444756E-79.69230722
TGAATGG2253.810419E-89.62
AGAGTGA1300.00113641589.34615410
Produced by FastQC (version 0.11.2)