FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_89.3520000005d59d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_89.3520000005d59d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA80.7843137254901961No Hit
GGCCTATCGGAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GTGAATGGGACCTATCGGAAAAAAAAAAAAAAA30.29411764705882354No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GCCTATCGGTTGAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
ATCCTATCGGTTGAACGTACTCTGCGTTGATAC20.19607843137254902No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT20.19607843137254902No Hit
GCCTATCGGTAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTTAATGGGAAGCAGTGGTA20.19607843137254902No Hit
TGCCTATCGGTTGAACGTACTCTGCGTTGATAC20.19607843137254902No Hit
GAGTGAATGGGGAGGCCTATCGGAAAAAAAAAA20.19607843137254902No Hit
CAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GCCTATCGGAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA20.19607843137254902No Hit
CTACTATACCTATCGGTTGAACGTACTCTGCGT20.19607843137254902No Hit
CCCAATCTCCTATCGGTTGAACGTACTCTGCGT20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)