FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_88.3520000005d573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_88.3520000005d573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27613
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA2280.8256980407778944No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA2100.7605113533480607No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA2010.7279180096331438No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1190.43095643356390106No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA1050.3802556766740304No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA960.3476623329591135No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT880.3186904718791873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.27885416289428894No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA760.27523268025929815No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA690.2498823018143628No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA650.23539637127439972No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA610.2209104407344367No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA560.20280302755948282No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA560.20280302755948282No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA520.1883170970195198No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC460.16658820120957518No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC450.16296671857458442No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA440.15934523593959365No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.15934523593959365No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA430.15572375330460292No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA430.15572375330460292No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAA410.1484807880346214No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC410.1484807880346214No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA400.1448593053996306No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA390.14123782276463984No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA370.1339948574946583No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA370.1339948574946583No Hit
GTGAATGGGGTCAAGCTCAAAAAAAAAAAAAAA370.1339948574946583No Hit
GGTATCAACGCAGAGTGAATGGGTCAAGCTCAA370.1339948574946583No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC370.1339948574946583No Hit
GTCAAGCTCAACACAAAAAAAAAAAAAAAAAAA360.13037337485966755No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.13037337485966755No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA340.12313040958968602No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA340.12313040958968602No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC330.11950892695469525No Hit
TATCAACGCAGAGTGAATGGGTCAAGCAAAAAA320.11588744431970449No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC310.11226596168471371No Hit
AGCTCAACACGTACTCAAAAAAAAAAAAAAAAA310.11226596168471371No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA300.10864447904972295No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA290.10502299641473219No Hit
TATCAACGCAGAGTGAATGGGGTCAAGCTCAAC290.10502299641473219No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA290.10502299641473219No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA290.10502299641473219No Hit
GCTCAACACGTACTAAAAAAAAAAAAAAAAAAA290.10502299641473219No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCA200.008448156527.01272617
CTATACC200.00852361426.9637013
CCTCGTC200.00852361426.9637015
CTCGTCA404.9026904E-420.2227766
GATACCA350.00532821419.3299527
TATCAAC2500.015.6673821
TCTGCGT4950.015.58111227
ATCAACG2550.014.8305172
TCAACGC2900.013.9467433
GTATCAA1756.184564E-1113.1204671
TCGTCAA853.813564E-412.6888027
CTCTGCG6400.012.05101626
CAACGCA3350.011.6708574
CGTCAAG3300.011.0306058
ACTCTGC7000.011.01807225
TACTCTG7350.010.8615924
GTACTCT7400.010.78820223
AACGCAG3750.010.4259645
CGTACTC8150.09.79542322
ACGTACT8400.09.16503221
Produced by FastQC (version 0.11.2)