FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_87.3520000005d566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_87.3520000005d566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46877
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1490.3178531049341895No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1260.2687885316893146No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA960.2047912622394778No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT750.159993173624592No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA730.1557266889946029No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC660.140793992789641No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT630.1343942658446573No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.13012778121466817No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA570.12159481195468994No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT510.10879535806472257No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA470.10026238880474433No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA470.10026238880474433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCT200.008537604526.993596
ATCTACG200.008537604526.993592
TCTACGC200.008537604526.993593
CTACGCA200.008537604526.993594
GGGGCAA200.008537604526.9935918
TATCTAC200.00858227826.9647831
ACTAAGT300.00223563622.4946614
TGACTAA300.00223563622.4946612
TAAGTAC300.00223563622.4946616
CTAAGTA300.00223563622.4946615
GCTGACT300.00223563622.4946610
CTGACTA350.005463066519.28113611
TGCGTTG2900.018.63617927
AATGGGT552.0954998E-417.1777384
GCAGCTG1251.5079422E-915.116417
TATCAAC2250.014.3812171
TCTGCGT9550.013.15753327
ATGGGGC853.867607E-412.70286617
ATCAACG2550.012.7028662
ACAGCTG1501.960143E-812.5970097
Produced by FastQC (version 0.11.2)