FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_86.3520000005d54d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_86.3520000005d54d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53238
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT4590.862166121942973No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2370.4451707427025809No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA1890.35500957962357715No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1790.33622600398211805No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC1680.31556407077651305No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA1370.2573349862879898No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1300.24418648333896842No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC1270.23855141064653065No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA1230.23103798038994705No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1210.22728126526165524No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC1180.22164619256921747No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1170.21976783500507158No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG1020.19159247154288292No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA960.18032232615800742No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC930.1746872534655697No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA930.1746872534655697No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA920.17280889590142381No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC830.1559036778241106No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA830.1559036778241106No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA830.1559036778241106No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA780.14651189000338102No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA770.14463353243923513No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA750.14087681731094331No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA750.14087681731094331No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA710.13336338705435968No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA670.12584995679777602No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA640.12021488410533829No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA630.11833652654119238No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA630.11833652654119238No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA620.11645816897704647No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA590.11082309628460874No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA580.10894473872046283No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA570.10706638115631692No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC560.10518802359217101No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC550.10330966602802509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAG200.00841640527.07931924
CAAGGCT200.00841640527.07931927
CCAAGGC200.00841640527.07931926
TAAATTC200.00857289326.97742516
AAATTCC200.00857289326.97742517
TTCCGTC200.00857289326.97742520
AATTCCG200.00857289326.97742518
AAGGGGC200.00857289326.97742518
TCCGTCC200.00857289326.97742521
ATTCCGT200.00857289326.97742519
CAATCGG350.00549164919.2695875
AATCGGG350.00549164919.2695876
TATCAAC2350.014.923681
TGATACC550.004089900314.77053827
CGTGTTC657.530332E-414.5263057
CTGCAAA657.530332E-414.52630511
GGTATCA657.530332E-414.5263051
TCTGCGT7950.014.30605627
ATCAACG2300.014.0751762
TCAACGC2350.013.7757043
Produced by FastQC (version 0.11.2)