FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_84.3520000005d509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_84.3520000005d509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58520
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC2670.45625427204374575No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.23923444976076555No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA1290.22043745727956254No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA1160.1982228298017772No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA870.14866712235133286No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.12474367737525634No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11961722488038277No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT650.11107313738892688No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA620.10594668489405332No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA610.10423786739576213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.7574E-426.988884
ACGCTGA257.7574E-426.988881
GCTGAGT257.7574E-426.988883
TCGCACT200.00856203226.988885
TCTCACT257.7574E-426.988885
TGAGTGA257.7574E-426.988885
GTATCAT352.0126246E-423.1333241
CTGCACT352.0126246E-423.1333245
CGCTGAG300.002244553822.4907322
GTCACTG300.002244553822.4907327
ACTACTG553.807636E-722.081816
ATACCAC454.4623033E-521.02732527
TCGCAGA454.5217526E-520.9913527
TCAACTG404.963205E-420.2416616
ATCATCG404.963205E-420.2416613
ATCGCAG404.963205E-420.2416616
CCGCACT350.005484616419.2777715
GATACCA501.00343765E-418.92459326
TGATACC501.00343765E-418.92459325
TCTGCGT19900.018.61212527
Produced by FastQC (version 0.11.2)