FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_81.3520000005d49e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_81.3520000005d49e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24729
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1150.4650410449270088No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1130.4569533745804521No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA920.372032835941607No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA860.347769824901937No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA740.29924380282259694No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA690.2790246269562052No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA670.27093695660964856No Hit
GTACGTAACGAAAAAAAAAAAAAAAAAAAAAAA650.2628492862630919No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA640.25880545108981357No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC590.23858627522342188No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA580.23454244005014355No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC580.23454244005014355No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA550.22241093453030852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.20219175866391684No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA490.1981479234906385No Hit
AGCCATTCCGTTACGTACTCTGCGTTGATACCA480.19410408831736017No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT460.1860164179708035No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT430.1738849124509685No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA430.1738849124509685No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA390.15770957175785513No Hit
GTGAATGGGAGTACGTAACGAAAAAAAAAAAAA380.1536657365845768No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC360.14557806623802014No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTAACG350.1415342310647418No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.13749039589146345No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA320.12940272554490678No Hit
GGAGTACGTAACGAAAAAAAAAAAAAAAAAAAA300.1213150551983501No Hit
GAGTACGTAACGGAAAAAAAAAAAAAAAAAAAA300.1213150551983501No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA290.11727122002507177No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA280.11322738485179343No Hit
GTGAATGGGGTACGTAACGGAAAAAAAAAAAAA280.11322738485179343No Hit
GTGAATGGGGAGTACGTAACGGAAAAAAAAAAA270.1091835496785151No Hit
GAGTGAATGGGGAGTACGTAACGAAAAAAAAAA260.10513971450523676No Hit
GTGAATGGGAGTACGTAACGGAAAAAAAAAAAA260.10513971450523676No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA250.10109587933195842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTGA2750.018.68397527
TATCAAC1850.017.5056361
ATCAACG2100.014.7790632
ATGGGGT550.004099235414.72064712
TCAACGC2150.014.4353633
CAACGCA2450.013.2185414
GTTGATA800.003440406611.83113725
TTAGCCA800.003493333511.8071864
TGCGTTG4650.011.04966426
AAGCCAT1503.2553526E-610.7951426
AACGCAG2950.010.520695
TGATACC900.00827776110.51656627
TTGATAC900.00827776110.51656626
CGTTGAT900.00827776110.51656624
CTGCGTT5650.09.57260126
TCTGCGT8350.08.58238127
CAGAGTG3807.094059E-118.1673774
AGAGTGA3859.276846E-118.0613075
GCAGAGT4052.746674E-107.6632183
CAGCCAT4950.07.63292848
Produced by FastQC (version 0.11.2)