FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_80.3520000005d474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_80.3520000005d474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48256
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC7031.4568136604774538No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA5121.0610079575596816No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA4760.9864058355437666No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA3650.756382625994695No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA3130.6486240053050398No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA3060.6341180371352786No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA2890.5988892572944297No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA2820.5843832891246684No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA2370.4911306366047746No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA2240.4641909814323607No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA2210.4579741379310344No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA2070.428962201591512No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.4248176392572944No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1830.3792274535809019No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT1780.3688660477453581No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA1780.3688660477453581No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1560.3232758620689655No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1510.31291445623342173No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1470.3046253315649867No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1470.3046253315649867No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1430.2963362068965517No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2963362068965517No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC1380.285974801061008No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1290.2673242705570292No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1290.2673242705570292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.25074602122015915No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1160.2403846153846154No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1130.2341677718832891No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.21966180371352784No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA1030.2134449602122016No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1000.20722811671087535No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1000.20722811671087535No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA970.20101127320954906No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA940.1947944297082228No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC920.1906498673740053No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA920.1906498673740053No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA900.1865053050397878No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA900.1865053050397878No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA900.1865053050397878No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA830.17199933687002653No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC770.159565649867374No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA770.159565649867374No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA760.15749336870026526No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA750.1554210875331565No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG740.15334880636604775No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA740.15334880636604775No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA740.15334880636604775No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA720.14920424403183022No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA670.13884283819628646No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA660.13677055702917773No Hit
GTATCATCGCAGAGTGAATGGGGCTAACTATCC640.1326259946949602No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA620.12848143236074272No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA590.12226458885941645No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA590.12226458885941645No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA580.1201923076923077No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA570.11812002652519893No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA540.11190318302387268No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA540.11190318302387268No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA510.10568633952254643No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA510.10568633952254643No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA500.10361405835543767No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA500.10361405835543767No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA500.10361405835543767No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA500.10361405835543767No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA490.10154177718832891No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA490.10154177718832891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.755311E-426.9781954
GCTGAGT257.755311E-426.9781953
TGAGTGA257.755311E-426.9781955
GTATGGG200.00856381326.9781933
GTGTATG200.00856381326.9781931
CCTTGGC200.00856381326.9781933
TATCATC405.920865E-726.9781932
TGTATGG200.00856381326.9781932
TCTACGC551.3875251E-824.525633
CTACGCA551.3875251E-824.525634
GTATCAT451.6536451E-623.9806161
TATCTAC653.2268872E-922.8277031
ACGCTGA300.002243997522.4818291
ATCTACG603.4944605E-822.4818292
TACGCAG603.4944605E-822.4818295
TCGCAGA504.1299863E-621.5825567
ATCGCAG504.1299863E-621.5825566
TCATCGC504.1299863E-621.5825564
GGTATCA751.6800186E-819.7840081
ATCATCG559.422722E-619.6205043
Produced by FastQC (version 0.11.2)