FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_8.3520000005cb8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_8.3520000005cb8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA242.3529411764705883No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC131.2745098039215685No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA111.0784313725490196No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA100.9803921568627451No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA80.7843137254901961No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC60.5882352941176471No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA60.5882352941176471No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA60.5882352941176471No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA50.49019607843137253No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA50.49019607843137253No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GTGAATGGGCATGAGCAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA40.39215686274509803No Hit
CGCATGAGCACTGTACGTACTCTGCGTTGATAC40.39215686274509803No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA40.39215686274509803No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA30.29411764705882354No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29411764705882354No Hit
GGCATGAGCACTGTACGTACTCTGCGTTGATAC30.29411764705882354No Hit
CCTGCCATGAGCACTGTACGTACTCTGCGTTGA20.19607843137254902No Hit
GCCCCCATGAGCACTGTACGTACTCTGCGTTGA20.19607843137254902No Hit
GTCCAGCATGAGCACTGTACGTACTCTGCGTTG20.19607843137254902No Hit
GAATGGGCACTGTACGTACCTGCAAAAAAAAAA20.19607843137254902No Hit
CCCCCCGCCATGAGCACTGTACGTACTCTGCGT20.19607843137254902No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGTACCGCAAAAAAAAAAA20.19607843137254902No Hit
ACGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACCTAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
ACGCAGAGTGAATGGGCACTGTACAAAAAAAAA20.19607843137254902No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGTAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACGACAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA20.19607843137254902No Hit
TATCATCGCAGAGTGAATGGGCACTGTACGTAC20.19607843137254902No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA20.19607843137254902No Hit
TACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA20.19607843137254902No Hit
AACGCAGAGTGAATGGGCACTGTACGTACCAAA20.19607843137254902No Hit
GGCACTGTACGTACTCAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTACTACCAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GCCACCATGAGCACTGTACGTACTCTGCGTTGA20.19607843137254902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA20.19607843137254902No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA20.19607843137254902No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA20.19607843137254902No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA20.19607843137254902No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)