FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_75.3520000005d3d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_75.3520000005d3d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38536
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA920.23873780361220676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1998131617189122No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT650.16867344820427652No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA590.15310359144695868No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA550.14272368694208012No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA520.13493875856342122No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA520.13493875856342122No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA470.12196387793232302No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA460.11936890180610338No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA450.11677392567988375No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA420.10898899730122484No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA400.10379904504878555No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA390.1012040689225659No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA390.1012040689225659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG306.983354E-527.0000024
ACGCTGA306.983354E-527.0000021
TGAGTGA306.983354E-527.0000025
CAACTCA200.00850922427.01
ATCTACG257.6904567E-426.9999982
GTATCAT257.6904567E-426.9999981
TCTACGC257.6904567E-426.9999983
TACGCAG257.6904567E-426.9999985
TATCTAC257.6904567E-426.9999981
TATCATC257.6904567E-426.9999982
CTACGCA257.6904567E-426.9999984
ATCGCAG257.6904567E-426.9999986
TCATCGC257.6904567E-426.9999984
CATCGCA257.6904567E-426.9999985
GACTCGT401.7940685E-523.6256
GCTGAGT351.9897036E-423.1428593
TCGCAGA300.002225393422.5000027
CGCTGAG404.9072236E-420.252
ATCATCG350.005438305419.2857153
GCGTTGA906.6484063E-918.027
Produced by FastQC (version 0.11.2)