FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_74.3520000005d3b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_74.3520000005d3b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55040
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6961.2645348837209303No Hit
GACACAGACAGTACGTACTCTGCGTTGATACCA6741.224563953488372No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA4630.8412063953488372No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA4620.8393895348837209No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA4600.8357558139534883No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT4520.8212209302325582No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3390.6159156976744187No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA3340.6068313953488372No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA3280.5959302325581396No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA2880.5232558139534884No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA2800.5087209302325582No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2680.4869186046511628No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA2220.40334302325581395No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA2220.40334302325581395No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA2190.3978924418604651No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA2050.37245639534883723No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA1970.357921511627907No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.345203488372093No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1880.34156976744186046No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA1700.30886627906976744No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA1640.2979651162790698No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA1610.2925145348837209No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA1570.28524709302325585No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA1560.28343023255813954No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA1540.279796511627907No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1490.2707122093023256No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT1480.26889534883720934No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA1470.267078488372093No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA1440.2616279069767442No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA1370.24890988372093023No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA1280.23255813953488372No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.2271075581395349No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA1230.2234738372093023No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA1220.22165697674418602No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA1210.21984011627906977No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1200.2180232558139535No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA1200.2180232558139535No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT1140.2071220930232558No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA1070.19440406976744187No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.19440406976744187No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA1040.18895348837209303No Hit
GTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.18531976744186046No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1010.18350290697674418No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA1000.1816860465116279No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA980.17805232558139536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.17623546511627905No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.1744186046511628No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA950.1726017441860465No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA950.1726017441860465No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA940.17078488372093023No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA880.15988372093023256No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA870.15806686046511628No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA860.15625No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.1471656976744186No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.14353197674418605No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA780.14171511627906977No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA780.14171511627906977No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG780.14171511627906977No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.14171511627906977No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA770.1398982558139535No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA760.1380813953488372No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA760.1380813953488372No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA760.1380813953488372No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.13444767441860467No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA720.1308139534883721No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA720.1308139534883721No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA710.12899709302325582No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA700.12718023255813954No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA700.12718023255813954No Hit
GTGAATGGGACACAGACAGTACGTACTCAAAAA690.12536337209302326No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA690.12536337209302326No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC660.11991279069767442No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA630.11446220930232559No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA610.11082848837209301No Hit
CAACTCAGAGTGAATGGGACACAGACAGTAAAA610.11082848837209301No Hit
CAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA600.10901162790697674No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA590.10719476744186048No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA580.10537790697674419No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA570.1035610465116279No Hit
GGACACAGACAGTACAAAAAAAAAAAAAAAAAA560.10174418604651163No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACTC200.00854742726.9954473
GTATCAT1000.026.9708821
ATCAACT200.0085855326.970882
CACTGCT751.8189894E-1225.24172827
CCACTGC751.8189894E-1225.24172826
TATCATC1050.024.4022272
ACCACTG801.8189894E-1223.66411825
TACCACT801.8189894E-1223.66411824
TCTACGC1100.023.3142473
TACGCAG1100.023.3142475
CTACGCA1100.023.3142474
ATCTACG1100.023.2930342
TATCTAC1100.023.2930341
CAACTCA1100.022.0670851
ATACCAC901.4551915E-1121.03477323
ATCGCAG1300.020.7657286
CATCGCA1300.020.7657285
ATCATCG1250.020.5165393
TCATCGC1250.020.5165394
GGTATCA404.980341E-420.228161
Produced by FastQC (version 0.11.2)