FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_72.3520000005d382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_72.3520000005d382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33732
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC3370.9990513459030002No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC3050.904185936203012No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC1180.3498161982687063No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC1110.3290643898968339No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1020.30238349341871223No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1010.29941894936558755No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.27570259694059057No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA910.26977350883434126No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA880.2608798766749674No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA840.24902170046246885No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC800.23716352424997034No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA770.22826989209059648No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC730.21641171587809793No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT710.21048262777184867No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA700.20751808371872407No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC600.17787264318747778No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC600.17787264318747778No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC600.17787264318747778No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA580.17194355508122852No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA560.16601446697497924No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA550.1630499229218546No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA490.14526265860310683No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT490.14526265860310683No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA490.14526265860310683No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA470.13933357049685757No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA460.13636902644373294No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC450.13340448239060831No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA410.1215463061781098No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA410.1215463061781098No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA410.1215463061781098No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC400.11858176212498517No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC390.11561721807186054No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA390.11561721807186054No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC390.11561721807186054No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC370.1096881299656113No Hit
TGTCCACACTACACACGTACTCTGCGTTGATAC350.10375904185936204No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA350.10375904185936204No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC340.1007944978062374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA306.863534E-527.05365226
ATACCAC404.8240344E-420.2902427
TTGATAC2000.019.613927
GTTGATA2150.018.24548526
TGATACC500.002158235516.23219125
TATCAAC1201.3915269E-814.6322551
CACTCCA852.3767767E-514.2799586
CGTTGAT2850.013.76413825
TATCCAC600.007401203313.4866287
AGTCCAC600.007401203313.4866287
ACCTCCA750.00219021212.5875186
TCTGCGT11000.012.42008527
TCAACGC1359.547657E-711.9881123
AATGGGT906.218871E-411.9881124
ATCAACG1359.547657E-711.9881122
GCGTTGA3400.011.93543524
CAACGCA1452.2600925E-611.1613474
CTCTGCG13100.010.42907926
ACTCCAC1853.4956793E-710.2060967
ATCCACA2656.0754246E-109.6696588
Produced by FastQC (version 0.11.2)