FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_70.3520000005d332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_70.3520000005d332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57260
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC6601.1526370939573873No Hit
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA4560.7963674467341949No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA3950.689835836535103No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA2830.49423681453021306No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA2600.4540691582256374No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA2520.4400977995110024No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA2350.4104086622424031No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA1900.33181976947258124No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC1890.3300733496332518No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.3091163115612993No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA1750.3056234718826406No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA1650.28815927348934683No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA1650.28815927348934683No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC1540.26894865525672373No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA1530.2672022354173943No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA1490.2602165560600769No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC1440.25148445686342996No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.23402025847013624No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC1250.21830247991617185No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA1250.21830247991617185No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC1240.21655606007684244No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA1160.20258470136220746No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1120.19559902200488996No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA1090.19035976248690184No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA1070.1868669228082431No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA1040.18162766329025498No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA1010.17638840377226686No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA1010.17638840377226686No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA960.16765630457561997No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA960.16765630457561997No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC920.16067062521830247No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA910.1589242053789731No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA880.153684945860985No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT830.1449528466643381No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCAAAAA820.14320642682500873No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC820.14320642682500873No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA820.14320642682500873No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG790.1379671673070206No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA760.13272790778903248No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA750.1309814879497031No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA740.12923506811037372No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.12748864827104436No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA680.11875654907439748No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA670.11701012923506812No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGAAA660.11526370939573874No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA650.11351728955640936No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA640.11177086971707999No Hit
AACGCAGAGTGAATGGGGTAGTTCTGACACGTA640.11177086971707999No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA620.10827803003842122No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA610.10653161019909187No Hit
GTATCATCGCAGAGTGAATGGGGTAGTTCTGAC610.10653161019909187No Hit
GACACGTACAAAAAAAAAAAAAAAAAAAAAAAA580.10129235068110373No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA580.10129235068110373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCT200.00849339427.032422
GCGTTTA200.00849339427.032423
ACGCTGA356.4234428E-627.0087491
ACACTTA257.721995E-427.00874918
ACTTACT257.721995E-427.00874920
GTATCTA307.0265756E-527.0087471
TGACACT257.761634E-426.9851416
GTATCAT950.025.5872341
CGCTGAG401.8217957E-523.6119982
TATCATC1100.023.3053472
ATCATCG1050.023.1301213
TCATCGC1050.023.1301214
CATCGCA1050.023.1301215
TCGCAGA950.022.7243297
CTTACTC300.0022343722.5072921
GACACTT300.002245777822.48761617
ATCGCAG1000.021.5881126
CAACTCA658.12106E-820.7759591
GGCGGTA404.966055E-420.2388556
TGCGTTG6400.019.42953727
Produced by FastQC (version 0.11.2)