FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_7.3520000005cb63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_7.3520000005cb63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14405
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA910.6317250954529677No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA820.5692467893092676No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA600.4165220409580007No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA600.4165220409580007No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA560.388753904894134No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA550.3818118708781673No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA550.3818118708781673No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA430.2985074626865672No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA400.2776813606386671No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.24991322457480042No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA350.24297119055883376No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC280.194376952447067No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA280.194376952447067No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA270.1874349184311003No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA270.1874349184311003No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA270.1874349184311003No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA240.16660881638320027No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA240.16660881638320027No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA230.1596667823672336No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA230.1596667823672336No Hit
GTGAATGGGCATTGCTGAAGACGTAAAAAAAAA220.15272474835126693No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA220.15272474835126693No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC210.14578271433530024No Hit
GAGTGAATGGGCATTGCTGAAGACAAAAAAAAA200.13884068031933355No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA190.13189864630336687No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA180.12495661228740021No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA180.12495661228740021No Hit
GCTGAAGACGTAAAAAAAAAAAAAAAAAAAAAA180.12495661228740021No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG170.11801457827143352No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA170.11801457827143352No Hit
CCGCATTGCTGAAGACGTACTCTGCGTTGATAC160.11107254425546687No Hit
GCTGAAGACGAAAAAAAAAAAAAAAAAAAAAAA160.11107254425546687No Hit
GCATTGCTGAAGACGTACAAAAAAAAAAAAAAA160.11107254425546687No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA160.11107254425546687No Hit
GCATTGCTGAAGACGTAAAAAAAAAAAAAAAAA160.11107254425546687No Hit
CAACTCAGAGTGAATGGGCATTGCTGAAAAAAA160.11107254425546687No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA150.10413051023950018No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA150.10413051023950018No Hit
GTGAATGGGCATTGCTGAAGACGAAAAAAAAAA150.10413051023950018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC802.9256626E-818.6080131
TCTGCGT3850.017.16414327
TCAACGC806.4211054E-716.857643
ATCAACG806.4211054E-716.857642
GCGCATT603.8524464E-415.7337957
TGGGGCA550.00400437214.7121216
CAACGCA953.8554954E-614.1959074
GCGTTGA903.763883E-513.48611127
CTCTGCG5000.013.21638926
CGCATTG2700.012.487148
ACTCTGC5400.012.28003625
CCGCATT1001.0116758E-412.13757
TACTCTG5450.011.91906124
ACGCAGA1851.713488E-911.7043051
GTACTCT5600.011.599823
AACGCAG1051.5953404E-411.5595255
AGCATTG1204.2787862E-511.2384258
CGTACTC6150.011.00252122
CTGCGTT1351.1335127E-510.98868426
CGCAGAG1852.379602E-810.9346862
Produced by FastQC (version 0.11.2)