FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_67.3520000005d2e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_67.3520000005d2e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45315
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC4480.9886351097870462No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.7326492331457575No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA3290.726028908749862No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA2790.6156901688182721No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA2770.6112766192210085No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA2340.5163853028798411No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA2300.5075582036853139No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1930.4259075361359373No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA1930.4259075361359373No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1750.38618558976056494No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA1660.36632461657287874No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA1630.35970429217698335No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1620.35749751737835156No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA1580.3486704181838243No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1410.3111552466070837No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA1380.30453492221118833No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1280.28246717422487033No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA1270.28026039942623854No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA1220.26922652543307957No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA1200.264812975835816No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA1190.2626062010371842No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1170.25819265143992054No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA1130.24936555224539333No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA1110.24495200264812977No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC1090.24053845305086619No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC1080.23833167825223436No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1000.22067747986317995No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA990.21847070506454813No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA990.21847070506454813No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT850.18757585788370296No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA840.18536908308507116No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA820.18095553348780755No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA810.17874875868917578No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA810.17874875868917578No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA800.17654198389054399No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.16992165949464857No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA770.16992165949464857No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA760.16771488469601675No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA760.16771488469601675No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA740.1633013350987532No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA720.15888778550148958No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA720.15888778550148958No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA710.15668101070285778No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA690.15226746110559417No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA690.15226746110559417No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT680.15006068630696237No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG670.14785391150833058No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA660.14564713670969878No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA630.13902681231380337No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA620.13682003751517158No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA610.13461326271653978No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA600.132406487917908No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.13019971311927617No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAAAAAA570.12578616352201258No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA570.12578616352201258No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA560.12357938872338077No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.12137261392474899No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA500.11033873993158998No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA490.1081319651329582No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA490.1081319651329582No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA490.1081319651329582No Hit
CCCCTAATGCGCTCTGAACGTACTCTGCGTTGA470.10371841553569458No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA470.10371841553569458No Hit
TATCAACGCAGAGTGAATGGGCTCAAAAAAAAA460.10151164073706279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA200.00852478227.011
CGTTAAA200.00852478227.013
CAACTCA356.400749E-627.01
GCGTTAA200.00852478227.012
TCATAAT200.00852478227.05
GTTAAAA200.00852478227.014
GTATCAT504.969479E-926.9999981
TACGCAG751.8189894E-1225.25
TATCTAC751.8189894E-1225.21
ATCTACG701.0913936E-1125.0714282
TCTACGC701.0913936E-1125.0714283
CTACGCA701.0913936E-1125.0714284
TATCATC551.3709723E-824.5454542
GGTATCA451.6383929E-624.01
CATCGCA653.1832315E-922.8461555
CGTAGAG300.002231415122.53
ATCATCG603.4529876E-822.53
TCATCGC603.4529876E-822.54
TCGCAGA707.492417E-921.2142877
ATCGCAG707.492417E-921.2142876
Produced by FastQC (version 0.11.2)