FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_65.3520000005d290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_65.3520000005d290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18847
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA1660.8807767814506288No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1490.7905767496153234No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1250.6632355282007747No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1190.6314002228471375No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA840.44569427495092057No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA830.4403883907253144No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC810.42977662227410196No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC770.4085530853716772No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA760.40324720114607093No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA750.3979413169204648No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA730.3873295484692524No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC600.31835305353637183No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC590.31304716931076565No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA510.27060009550591607No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA410.21754125324985407No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT360.1910118321218231No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA360.1910118321218231No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC360.1910118321218231No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA320.1697882952193983No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC310.16448241099379213No Hit
ACGCAGAGTACGTAAAAAAAAAAAAAAAAAAAA290.1538706425425797No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC290.1538706425425797No Hit
CGTTACGTACTCAAAAAAAAAAAAAAAAAAAAA290.1538706425425797No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.14325887409136734No Hit
GTGAATGGGACATACGCAAAAAAAAAAAAAAAA260.13795298986576113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13264710564015492No Hit
CACATACGCACGTTACGTACTCTGCGTTGATAC240.12734122141454873No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA240.12734122141454873No Hit
GACATACGCAAAAAAAAAAAAAAAAAAAAAAAA230.12203533718894255No Hit
GTGAATGGGCATACGCAAAAAAAAAAAAAAAAA220.11672945296333634No Hit
ATCATACGCACGTTACGTACTCTGCGTTGATAC220.11672945296333634No Hit
GTCATACGCACGTTACGTACTCTGCGTTGATAC210.11142356873773014No Hit
GGCATACGCAAAAAAAAAAAAAAAAAAAAAAAA200.10611768451212396No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA200.10611768451212396No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC190.10081180028651773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATAC806.707178E-716.8533928
TATCAAC1900.014.2301751
TCTGCGT3450.013.75125927
ATCAACG2150.012.5420592
TCAACGC2251.8189894E-1211.9846333
ATGGGAC850.005444300411.1034115
CAACGCA2459.094947E-1211.0062964
ATGGGGT1000.001453825410.7861715
TCATACG1751.7672992E-610.015739
AACGCAG2706.366463E-119.9871955
AATGGGG2051.17541276E-79.8653994
CTCTGCG4900.09.6820126
GTGAATG5900.09.1651971
TGAATGG5700.08.7519362
GAATGGG5950.08.3842093
ACTCTGC5900.08.27073425
ACGCAGA3353.8580765E-98.049386
CGCAGAG3353.8580765E-98.049387
TGCGTTG1550.00557535337.87053627
CCATACG3805.638867E-107.8057818
Produced by FastQC (version 0.11.2)