FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_61.3520000005d219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_61.3520000005d219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41593
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA2800.6731902002740845No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC2060.4952756473445051No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC1760.4231481258865674No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA1530.36785035943548194No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA1470.35342485514389443No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA1430.34380785228283606No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1390.33419084942177774No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA1350.32457384656071936No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1320.3173610944149256No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC1020.24523357295698797No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.1803188036448441No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA720.1731060514990503No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA690.16589329935325656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.16589329935325656No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC660.1586805472074628No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1562762964921982No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA590.14185079220061067No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC540.12982953862428773No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA530.12742528790902316No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.12261678647849399No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA480.1154040343327002No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA470.11299978361743564No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT460.11059553290217103No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA450.10819128218690645No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA440.10578703147164185No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA430.10338278075637727No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA420.10097853004111268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCA306.996533E-527.04
ATCTACG257.7008246E-426.9999982
TCTACGC257.7008246E-426.9999983
TACGCAG257.7008246E-426.9999985
TATCTAC257.7008246E-426.9999981
GTATCAT350.0054454519.2857131
TATCATC350.0054454519.2857132
TGATACC703.7738191E-617.35714127
TGCGTTG4000.017.212527
ATGGGAA550.00415031414.7272725
TGGGGAC550.00415031414.7272726
CCACACC550.00415031414.7272727
ATGGTGT751.3305015E-414.4000016
GTATCAA1850.013.8648641
TTGATAC904.0800674E-513.50000126
TCTGCGT11150.013.07623327
ACTCTAT1451.2007149E-813.0344831
ATGGGGA1051.1992683E-512.8571435
TCTATGG1953.092282E-1112.4615383
CTGCGTT5850.012.46153826
Produced by FastQC (version 0.11.2)