FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_59.3520000005d1e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_59.3520000005d1e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56057
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACTGAGGCAAAAAAAAAAAAAAAA2480.44240683589917407No Hit
GCACTTACGTACTCAAAAAAAAAAAAAAAAAAA2210.3942415755391834No Hit
TATCAACGCAGAGTGAATGGGGCACTTACGTAC1780.31753393866956847No Hit
CTTACGTACTCAAAAAAAAAAAAAAAAAAAAAA1710.30504664894660793No Hit
GACTGAGGCAAAAAAAAAAAAAAAAAAAAAAAA1410.25152969299106265No Hit
GAATGGGACTGAGGCAAAAAAAAAAAAAAAAAA1380.24617799739550814No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCAAAA1340.2390424032681021No Hit
GTGAATGGGGCACTTACGTACTCAAAAAAAAAA1290.2301229106088446No Hit
GTGAATGGGACTGAGGCACAAAAAAAAAAAAAA1260.22477121501329003No Hit
GGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.22298731648143855No Hit
GGACTGAGGCAAAAAAAAAAAAAAAAAAAAAAA1180.210500026758478No Hit
ACGCAGAGTGAATGGGACTGAGGCAAAAAAAAA1010.18017375171700234No Hit
GAGTGAATGGGACTGAGGCAAAAAAAAAAAAAA1000.17838985318515083No Hit
GGACTGAGGCACAAAAAAAAAAAAAAAAAAAAA900.16055086786663575No Hit
GAACTGAGGCACAAAAAAAAAAAAAAAAAAAAA860.15341527373922972No Hit
GGCACTTACGTACTCAAAAAAAAAAAAAAAAAA860.15341527373922972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.1498474766755267No Hit
CTGAGGCACTTACGTACTCTGCGTTGATACCAC820.14627967961182367No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA790.14092798401626916No Hit
GTATCAACGCAGAGTGAATGGGGCACTTACGTA770.13736018695256613No Hit
GTGAATGGGGACTGAGGCAAAAAAAAAAAAAAA770.13736018695256613No Hit
GAATGGGACTGAGGCACAAAAAAAAAAAAAAAA770.13736018695256613No Hit
GACTGAGGCACAAAAAAAAAAAAAAAAAAAAAA710.12665679576145708No Hit
ACGCAGAGTGAATGGGGCACTTACGTACTCAAA680.12130510016590257No Hit
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11416950603849653No Hit
TATCAACGCAGAGTGAATGGGACTGAGGCACAA620.11060170897479352No Hit
TATCTACGCAGAGTGAATGGGGCACTTACGTAC600.10703391191109049No Hit
ACGCAGAGTGAATGGGACTGAGGCACAAAAAAA590.105250013379239No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG200.0084838627.037627
CTTCGTT200.0084838627.037626
CGCTGAG257.7525503E-426.9892772
CTGAGTG200.008558338526.9892774
ACGCTGA257.7525503E-426.9892771
AGGACTG307.058542E-526.9892776
ACGCATA200.008558338526.9892771
GTATCAT800.026.9892771
GAGTAAA200.008558338526.9892771
AGTAAAT200.008558338526.9892772
TGAGTGA200.008558338526.9892775
CAACTCA554.674803E-1026.9892751
GTTTCAA356.456361E-626.9892752
GGTTTCA356.456361E-626.9892751
TATCATC850.025.4016722
AACTCAG601.2823875E-924.7401712
ATCTACG551.3918907E-824.5357062
TCTACGC551.3918907E-824.5357063
TACGCAG551.3918907E-824.5357065
CTACGCA551.3918907E-824.5357064
Produced by FastQC (version 0.11.2)