FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_57.3520000005d1ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_57.3520000005d1ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40754
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC3910.9594150267458409No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1830.4490356774795112No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1590.3901457525641655No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1420.34843205574912894No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1300.3189870932914561No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1240.3042646120626196No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1100.2699121558620013No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA870.21347597781812827No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT790.19384600284634637No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA710.17421602787456447No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA670.1644010403886735No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC640.1570397997742553No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA620.1521323060313098No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA620.1521323060313098No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA590.14477106541689158No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC560.13740982480247338No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA550.13495607793100065No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA550.13495607793100065No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA540.13250233105952788No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA530.13004858418805518No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGAA520.12759483731658242No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA480.11777984983069147No Hit
GTCTACGTACCGTAACGTACTCTGCGTTGATAC470.11532610295921872No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC460.11287235608774598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10796486234480052No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGTA420.10305736860185502No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA420.10305736860185502No Hit
GCCCTACGTACCGTAACGTACTCTGCGTTGATA410.1006036217303823No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA410.1006036217303823No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA410.1006036217303823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCAGG200.00845801327.03694526
CACACTA200.00850906427.003695
CCTATAC200.00850906427.003692
CTATACC200.00850906427.003693
CGGGGCA200.00850906427.0036917
ACACTAC200.00850906427.003696
TCACTAC200.00850906427.003696
GGGGCAA200.00850906427.0036918
GGCCGTC200.00856035926.97051411
CCGTCGG200.00856035926.97051413
CGTCGGG200.00856035926.97051414
ACCTATA200.00856035926.9705141
GCCGTCG200.00856035926.97051412
TCGGGGC200.00856035926.97051416
GATACCA454.417046E-521.02873427
TGATACC952.4156907E-715.65296827
TATCAAC2350.014.9198591
TTGATAC1107.795461E-814.74742526
TCTGCGT9550.014.580132527
ATCAACG2400.014.0644232
Produced by FastQC (version 0.11.2)