FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_56.3520000005d184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_56.3520000005d184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34392
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA7962.314491742265643No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA3941.1456152593626425No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC3841.1165387299371947No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA2250.654221912072575No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2170.6309606885322168No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.616422423819493No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1840.535008141428239No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1750.5088392649453362No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA1730.5030239590602466No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA1680.48848569434752265No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA1510.43905559432426144No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1460.4245173296115376No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1220.3547336589904629No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC1080.31402651779483604No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA1030.29948825308211213No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA840.24424284717376132No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA810.235519888346127No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA810.235519888346127No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA800.23261223540358222No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC790.22970458246103745No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA750.21807397069085835No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC750.21807397069085835No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA730.2122586648057688No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC650.18899744126541054No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA600.17445917655268667No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA600.17445917655268667No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.17445917655268667No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA580.16864387066759712No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA580.16864387066759712No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA570.16573621772505234No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC560.16282856478250757No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.1599209118399628No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA540.15701325889741802No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA530.15410560595487324No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT520.15119795301232847No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA510.1482903000697837No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA510.1482903000697837No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA490.14247499418469411No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA470.13665968829960456No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA460.13375203535705976No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA450.130844382414515No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA450.130844382414515No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA440.1279367294719702No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG440.1279367294719702No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA440.1279367294719702No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA400.11630611770179111No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA390.11339846475924634No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA390.11339846475924634No Hit
CCCCCCCATGAGCACTGTACGTACTCTGCGTTG380.11049081181670156No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA370.10758315887415679No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA370.10758315887415679No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA370.10758315887415679No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG200.00843827327.0379561
ACGCTGA200.00843827327.0379561
TCAGAGT356.3441476E-626.9985435
GTAGAGT200.00849870426.998544
ACGTAGA200.00849870426.998542
CGTAGAG200.00849870426.998543
AACTCAG405.7989746E-726.998542
ACTCAGA405.7989746E-726.998543
CTCAGAG405.7989746E-726.998544
TATCATC200.00849870426.998542
TCGCAGA200.00849870426.998547
TAGAGTG200.00849870426.998545
ATCATCG200.00849870426.998543
ATCGCAG200.00849870426.998546
TCATCGC200.00849870426.998544
CAACTCA451.5992846E-624.0337371
GTATCAT300.002202339722.531631
TATCTAC454.3897628E-521.029521
ATCTACG454.4396482E-520.9988652
TCTACGC454.4396482E-520.9988653
Produced by FastQC (version 0.11.2)