FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_55.3520000005d16a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_55.3520000005d16a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47238
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCATTGCTGAAGA3810.8065540454718658No Hit
GCTGAAGACGTACTCAAAAAAAAAAAAAAAAAA2650.5609890342520852No Hit
GCATTGCTGAAGACGTACTCTGCGTTGATACCA2100.4445573478978788No Hit
GTGAATGGGCATTGCTGAAGAAAAAAAAAAAAA1650.34929505906261904No Hit
GCTGAAGACGTACAAAAAAAAAAAAAAAAAAAA1630.34506117955882976No Hit
GTGAATGGGCATTGCTGAAAAAAAAAAAAAAAA1580.33447648079935643No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACGTAC1420.3006054447690419No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.27943604725009524No Hit
GTATCAACGCAGAGTGAATGGGCATTGCTGAAG1240.26250052923493794No Hit
TATCAACGCAGAGTGAATGGGCATTGCTGAAAA1240.26250052923493794No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGAAAAAA1220.25826664973114866No Hit
GTGAATGGGCATTGCTGAAGACGTACTCAAAAA900.1905245776705195No Hit
ACGCAGAGTGAATGGGCATTGCTGAAAAAAAAA900.1905245776705195No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA820.17358905965536223No Hit
GCTGAAGACAAAAAAAAAAAAAAAAAAAAAAAA810.17147211990346756No Hit
GTGAATGGGCATTGCTGAAGACGTACAAAAAAA810.17147211990346756No Hit
GTGAATGGGCATTGCTGAAGACAAAAAAAAAAA760.16088742114399424No Hit
GCATTGCTGAAGACAAAAAAAAAAAAAAAAAAA750.1587704813920996No Hit
GCATTGCTGAAGAAAAAAAAAAAAAAAAAAAAA680.14395190312883693No Hit
GAGTGAATGGGCATTGCTGAAGAAAAAAAAAAA650.13760108387315298No Hit
GTATCATCGCAGAGTGAATGGGCATTGCTGAAG620.13125026461746897No Hit
ACGCAGAGTGAATGGGCATTGCTGAAGACAAAA610.12913332486557433No Hit
GCTGAAGACGTACTAAAAAAAAAAAAAAAAAAA590.12489944536178502No Hit
ATCAACGCAGAGTGAATGGGCATTGCTGAAGAC580.12278250560989035No Hit
TATCTACGCAGAGTGAATGGGCATTGCTGAAGA580.12278250560989035No Hit
GTGAATGGGGCATTGCTGAAAAAAAAAAAAAAA560.11854862610610102No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT550.11643168635420637No Hit
GTGAATGGGGCATTGCTGAAGAAAAAAAAAAAA550.11643168635420637No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGAAAAA530.11219780685041705No Hit
GAGTACGTCTTCAGCAAAAAAAAAAAAAAAAAA520.11008086709852237No Hit
GGTATCAACGCAGAGTGAATGGGCATTGCTGAA520.11008086709852237No Hit
GAGTGAATGGGCATTGCTGAAAAAAAAAAAAAA520.11008086709852237No Hit
TATCAACGCAGAGTGAATGGGGCATTGCTGAAG510.1079639273466277No Hit
AACGCAGAGTGAATGGGCATTGCTGAAGACGTA510.1079639273466277No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA510.1079639273466277No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.10584698759473306No Hit
AACGCAGAGTGAATGGGCATTGCTGAAAAAAAA500.10584698759473306No Hit
GCATTGCTGAAAAAAAAAAAAAAAAAAAAAAAA480.10161310809094373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG257.7052345E-427.0063927
TACGCAG405.8618025E-727.006395
ACGAGCA200.0085181627.006395
ATTGCAT200.0085181627.006396
CAACTCA200.0085625926.977661
ATCTACG356.449043E-626.977662
TCTACGC356.449043E-626.977663
AACTCAG200.0085625926.977662
TATCTAC356.449043E-626.977661
CGAGCAT351.9956157E-423.1483356
TAGAGTG658.050847E-820.7741455
GTAGAGT658.142342E-820.7520454
ACTGCAT404.9215904E-420.2547916
TAGCATT751.6572812E-819.8046867
CTACGCA559.416439E-619.6201154
ATTGCTT350.005448372619.29027711
TGCTTAA350.005448372619.29027713
TTGCTTA350.005448372619.29027712
AACGTAG701.7792263E-719.2697561
AACGCAT651.8655974E-618.696736
Produced by FastQC (version 0.11.2)