FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_54.3520000005d141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_54.3520000005d141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30552
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC3551.1619533909400368No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA1950.638256087981147No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1060.34694946321026443No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC1060.34694946321026443No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1010.33058392249279916No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA960.3142183817753339No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA860.28148730034040326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.2782141921969102No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA840.2749410840534171No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA720.2356637863315004No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT720.2356637863315004No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC660.216025137470542No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA640.20947892118355593No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA610.1996595967530767No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA600.19638648860958366No Hit
GAGTGAATGGGATCAGACGTACTCAAAAAAAAA560.1832940560356114No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA550.18002094789211837No Hit
GTATCAACGCAGAGTGAATGGGATCAGACGTAC490.16038229903116No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA490.16038229903116No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA470.15383608274417387No Hit
ACGCAGAGTGAATGGGATCAGACGTACTCAAAA470.15383608274417387No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT470.15383608274417387No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA460.1505629746006808No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA440.1440167583136947No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA390.12765121759622938No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA380.12437810945273632No Hit
GCATTGGATGGATGCCCGGGCATTGAGAAGGAA380.12437810945273632No Hit
GATCGTAGCAAAAAAAAAAAAAAAAAAAAAAAA350.11455878502225714No Hit
GTGAATGGGATCAGACGTACTCAAAAAAAAAAA350.11455878502225714No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA340.11128567687876408No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT330.108012568735271No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA330.108012568735271No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA320.10473946059177797No Hit
GTGAATGGGGGCGTGCAAAAAAAAAAAAAAAAA310.10146635244828489No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCAG200.00846889127.0082262
ATCATCG200.00846889127.0082263
TCATCGC200.00846889127.0082264
GTATCAT306.921316E-527.0082241
TATCATC306.921316E-527.0082242
ACGTAGC200.0085372726.9638758
TCGCAGA200.0085372726.9638757
CATCGCA200.0085372726.9638755
ATACCAC351.972196E-423.14990427
GATACCA450.00107460518.00548226
ATGGGAG751.3270235E-414.38073355
TATCAAC2100.014.1471651
ATGGGAA853.8341325E-412.6888835
TGATACC800.003505729411.81609827
TTGATAC800.003505729411.81609826
GTTGATA850.005513033411.12103525
TCAACGC2603.6379788E-1210.9071673
ATCAACG2603.6379788E-1210.9071672
CGTCTGA900.00843268210.5031981
TCTGCGT6550.010.30848227
Produced by FastQC (version 0.11.2)