FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate3_53.3520000005d11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate3_53.3520000005d11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG1130.4902811523776467No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA810.3514404720583131No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA650.28202013189864633No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT610.2646650468587296No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC590.25598750433877127No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC570.2473099618188129No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA480.20826102047900036No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA470.20392224921902116No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA470.20392224921902116No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.20392224921902116No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA470.20392224921902116No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA410.17788962165914612No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT390.1692120791391878No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC390.1692120791391878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.16053453661922942No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA340.14751822283929192No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC340.14751822283929192No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA330.14317945157931272No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT320.13884068031933355No Hit
GTTAAAAGATAACGCAGGTGTCCTAAGATGAGC280.12148559527941688No Hit
ACGCAGAGTGAATGGGTAGACGTAGAAAAAAAA270.1171468240194377No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT250.10846928149947933No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA250.10846928149947933No Hit
GAGCTACGTCTAAAAAAAAAAAAAAAAAAAAAA240.10413051023950018No Hit
ACTAGACGTAGCTCACGTACTCTGCGTTGATAC240.10413051023950018No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCT300.002200256622.49636812
GTTGATA751.2914003E-414.39767627
CGTTGAT1551.2551027E-1013.93323527
TATCAAC1755.638867E-1113.1695531
ACTAGAC750.00214003912.5979667
GCGTTGA1903.1759555E-911.36658626
ATCAACG1801.7924322E-811.2481842
TCAACGC1903.9808583E-810.6561753
CAACGCA2101.5214027E-810.2840554
TGCGTTG2152.1947926E-810.0448925
ACGCAGA3400.09.9683041
CGCAGAG3300.09.8165972
CAGAGTG3109.094947E-129.57914
GCAGAGT3109.094947E-129.57913
AGAGTGA3151.2732926E-119.427055
AACGCAG2152.43228E-79.4170855
GTATCAA1607.492259E-59.3203641
GAGTGAA3703.5288394E-108.0257316
AGTGAAT3703.5288394E-108.0257317
CTGCGTT2707.2856346E-77.998708724
Produced by FastQC (version 0.11.2)